PE anti-mouse CD254 (TRANCE, RANKL) Antibody

Pricing & Availability
IK22/5 (See other available formats)
Regulatory Status
Other Names
Tumor necrosis factor-related activation-induced cytokine, Receptor activator of NF-κB ligand (RANK L), Osteoprotegerin ligand (OPGL), Osteoclast differentiation factor (ODF), SOFA, TNFSF-11, CD254, RANKL
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
TRANCE transfected cells stained with IK22-5 PE
  • IK22/5
    TRANCE transfected cells stained with IK22-5 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
510005 50 µg 141€
Check Availability

Need larger quantities of this item?
Request Bulk Quote
510006 200 µg 291€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

CD254 is a 19 kD TNF superfamily member also known as TRANCE (TNF-related activation induced cytokine), RANK ligand, RANKL, TNFSF11, OPGL, and ODF. TRANCE is expressed on activated T cells and osteoclasts and has been implicated in the regulation of T cell and dendritic cell interactions as well as osteoclast differentiation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
NSO-derived recombinant mouse TRANCE
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK. Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2, and blocking of ligand binding1,2,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 510009-510014).

Application References
  1. Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
  2. Personal communication (Block IP WB)
  3. Gao Y, et al. 2007. J. Clin. Invest. 117:122.
  4. Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
  5. Fionda C, et al. 2007. J. Immunol. 178:4039.
  6. Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed
Product Citations
  1. Dai Y, et al. 2013. Mol Cancer Ther. 12:878. PubMed
  2. Takeshita S, et al. 2014. J Biol Chem. 289:16699. PubMed
  3. Ersching J, et al. 2016. PLoS Pathog. 12: 1005698. PubMed
  4. Dar HY, et al. 2018. Sci Rep. 8:2503. PubMed
  5. Dar HY, et al. 2018. Bone Rep. 8:46. PubMed
  6. Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed
  7. Gao Y, et al. 2007. J Clin Invest. 117:122. PubMed
AB_315553 (BioLegend Cat. No. 510005)
AB_2303587 (BioLegend Cat. No. 510006)

Antigen Details

TNF superfamily; 19 kD (Mammalian)

Activated T cells, osteoclasts

Regulates T and dendritic cell interactions, and osteoclast differentiation
Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
Cell Sources
Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts
Cell Targets
Dendritic cells, osteoclasts
Cell Type
Osteoclasts, T cells
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041.

Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement
Gene ID
21943 View all products for this Gene ID
View information about CD254 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD254 (TRANCE, RANKL)

  • PE anti-mouse CD254 (TRANCE, RANKL)

  • Purified anti-mouse CD254 (TRANCE, RANKL)

  • Ultra-LEAF™ Purified anti-mouse CD254 (TRANCE, RANKL)


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account