LEGEND MAX™ Mouse M-CSF ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, MCSF, M-CSF
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LEGEND-MAX_Mouse_M-CSF-ELISA-Kit
  • LEGEND-MAX_Mouse_M-CSF-ELISA-Kit
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448507 1 Pre-coated Plate 375€
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Description

As a potent hematopoietic cytokine, M-CSF is involved in diverse processes, such as the regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. M-CSF (macrophage colonystimulating factor) is a potent lineage restricted hematopoietic growth factor and member of the CSF family of hormone-like glycoproteins. It plays a key role in the regulation of cellular undifferentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.

BioLegend’s LEGEND MAX™ Mouse M-CSF ELISA kit is a Sandwich Enzyme-Linked
Immunosorbent Assay (ELISA) with a 96-well strip plate. The plate is pre-coated
with a monoclonal capture antibody. The detection antibody is a biotinylated
monoclonal antibody. This kit is specifically designed for the accurate quantitation
of mouse M-CSF in cell culture supernatant, serum, and plasma. It is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse M-CSF Pre-coated 96-well Strip Microplate
  • Mouse M-CSF Detection Antibody (200X)
  • Mouse M-CSF Standard
  • Avidin HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA

Sensitivity
6.1 ± 1.7 pg/mL
Standard Range
31.25 – 2000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 μL to 1,000 μL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Polypropylene tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
12977 View all products for this Gene ID
UniProt
View information about M-CSF on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 06-08-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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