ELISA MAX™ Deluxe Set Mouse M-CSF

Pricing & Availability
Regulatory Status
RUO
Other Names
colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
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  • ELISA-MAX_Deluxe_Mouse_M-CSF_082421.png
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448504 5 plates 229€
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Description

As a potent hematopoietic cytokine, M-CSF is involved in diverse processes, such as regulation of atherosclerosis or brain, bone resorption, placental development, and inflammatory responses. M-CSF (macrophage colonystimulating factor) is a potent lineage restricted hematopoietic growth factor and member of the CSF family of hormone-like glycoproteins. It plays a key role in the regulation of cellular undifferentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.

BioLegend’s ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The kit contains a monoclonal capture antibody and biotinylated monoclonal antibody specific for mouse M-CSF. BioLegend’s ELISA MAX™ Deluxe Set contains the components necessary for the accurate quantification of natural and recombinant mouse M-CSF. The set is designed for cost-effective and accurate quantification of mouse M-CSF in cell culture supernatant, serum, plasma or other biological fluids. BioLegend’s ELISA MAX™ Deluxe Sets are sensitive, accurate, and robust.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse M-CSF ELISA MAX™ Capture Antibody (200X)
  • Mouse M-CSF ELISA MAX™ Detection Antibody (200X)
  • Mouse M-CSF Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer B (5X)
  • Assay Diluent B (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
3.52 - 4.50 pg/mL
Standard Range
15.6 - 1000 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20). BioLegend Cat. No. 421601 is recommended.
  • Stop Solution (e.g. 2N H2SO4) BioLegend Cat. No. 423001 is recommended
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
NA
UniProt
View information about M-CSF on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 2    Revision Date: 09-20-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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