Brilliant Violet 510™ anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M170_BV510_CD11b_Antibody_FC_082512
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 510™. Data shown was gated on the myeloid cell population.
  • M170_BV510_CD11b_Antibody_FC_082512
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 510™. Data shown was gated on the myeloid cell population.
  • M170_BV510_CD11b_Antibody_ICC_121319
    C57 mouse bone marrow cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml of CD11b (clone M1/70) Brilliant Violet 510™ (red) and 10 µg/ml of CD45 (clone 30-F11) Alexa Fluor® 647 (blue) for 30 minutes at room temperature. The image was captured by 60X objective.
Compare all formats See Brilliant Violet 510™ spectral data See high resolution IF data...
Cat # Size Price Quantity Check Availability Save
101245 125 µL 132€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101263 50 µg 216€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee, Baboon, Rabbit
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
µg size: 0.2 mg/mL
µL size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µL size, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.4 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Karsten C, et al. 2015. J Immunol. 194:1841. PubMed
  2. Cardoso M, et al. 2015. Infect Immun . 83: 3534-3544. PubMed
  3. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  4. Woods T, et al. 2016. J Virol. 90: 2783 - 2793. PubMed
  5. Peterson T, et al. 2016. Proc Natl Acad Sci U S A. 113: 4470-4475. PubMed
  6. Kloepper J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4476-4481. PubMed
  7. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  8. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  9. Vicetti Miguel R, et al. 2016. PLoS One. 11: 0162445. PubMed
  10. Ender F, et al. 2017. PLoS One. 12(2):e0172446. PubMed
  11. Wang Y, et al. 2020. Cell Death Dis. 1.169444444. PubMed
  12. El Amki M, et al. 2020. Cell Rep. 33:108260. PubMed
  13. Kerkhofs D, et al. 2020. Theranostics. 7.022222222. PubMed
  14. Burns JC, et al. 2020. eLife. 9:00. PubMed
  15. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  16. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  17. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  18. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  19. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  20. Affo S, et al. 2021. Cancer Cell. 39(6):866-882.e11. PubMed
  21. Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed
  22. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  23. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  24. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  25. LaMothe RA et al. 2018. Front Immunol. 0.570138889 . PubMed
  26. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  27. Le TM et al. 2018. Journal of neurochemistry. 145(2):139-153 . PubMed
  28. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  29. Gentek R, et al. 2018. Immunity. 48:1160. PubMed
  30. Nakamoto N, et al. 2019. Nat Microbiol. 4:492. PubMed
  31. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  32. Eremenko E, et al. 2019. EBioMedicine. 43:424. PubMed
  33. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  34. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  35. Christ A, et al. 2018. Cell. 172:162. PubMed
  36. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  37. Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed
  38. Green DP, et al. 2019. Neuron. 101:412. PubMed
  39. McDonald LT, et al. 2017. PLoS One. 12:e0180724. PubMed
  40. Lee C, et al. 2020. Front Immunol. 11:77. PubMed
  41. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  42. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  43. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  44. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
  45. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  46. Briukhovetska D, et al. 2020. Frontiers in Immunology. 1.428472222. PubMed
  47. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  48. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  49. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
RRID
AB_2561390 (BioLegend Cat. No. 101245)
AB_2629529 (BioLegend Cat. No. 101263)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD11b Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD11b M1/70 FC
Biotin anti-mouse/human CD11b M1/70 FC
FITC anti-mouse/human CD11b M1/70 FC
PE anti-mouse/human CD11b M1/70 FC, IHC
PE/Cyanine5 anti-mouse/human CD11b M1/70 FC
Purified anti-mouse/human CD11b M1/70 FC, CyTOF®, IP, IHC-F, ICC
PE/Cyanine7 anti-mouse/human CD11b M1/70 FC
Alexa Fluor® 488 anti-mouse/human CD11b M1/70 FC, IHC-F, 3D IHC, SB
Alexa Fluor® 647 anti-mouse/human CD11b M1/70 FC, 3D IHC
Alexa Fluor® 700 anti-mouse/human CD11b M1/70 FC
Pacific Blue™ anti-mouse/human CD11b M1/70 FC
APC/Cyanine7 anti-mouse/human CD11b M1/70 FC
PerCP/Cyanine5.5 anti-mouse/human CD11b M1/70 FC
PerCP anti-mouse/human CD11b M1/70 FC
Brilliant Violet 421™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 570™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 605™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 650™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 711™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 785™ anti-mouse/human CD11b M1/70 FC
Brilliant Violet 510™ anti-mouse/human CD11b M1/70 FC, ICC
Ultra-LEAF™ Purified anti-mouse/human CD11b M1/70 FC, CyTOF®, IP, Block, Depletion, IHC-F, ICC
Purified anti-mouse/human CD11b (Maxpar® Ready) M1/70 FC, CyTOF®
Alexa Fluor® 594 anti-mouse/human CD11b M1/70 IHC-F, FC
PE/Dazzle™ 594 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 750 anti-mouse/human CD11b M1/70 FC
TotalSeq™-A0014 anti-mouse/human CD11b M1/70 PG
Brilliant Violet 750™ anti-mouse/human CD11b M1/70 FC
TotalSeq™-B0014 anti-mouse/human CD11b M1/70 PG
TotalSeq™-C0014 anti-mouse/human CD11b M1/70 PG
Spark NIR™ 685 anti-mouse/human CD11b M1/70 FC
PE/Fire™ 640 anti-mouse/human CD11b M1/70 FC
Spark YG™ 593 anti-mouse/human CD11b M1/70 FC
Spark YG™ 570 anti-mouse/human CD11b M1/70 IHC-F
PE/Fire™ 810 anti-mouse/human CD11b M1/70 FC
APC/Fire™ 810 anti-mouse/human CD11b Antibody M1/70 FC
Spark Blue™ 550 anti-mouse/human CD11b M1/70 FC
Spark UV™ 387 anti-mouse/human CD11b M1/70 FC
Go To Top Version: 3    Revision Date: 07-08-2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse/human CD11b

  • Biotin anti-mouse/human CD11b

  • FITC anti-mouse/human CD11b

  • PE anti-mouse/human CD11b

  • PE/Cyanine5 anti-mouse/human CD11b

  • Purified anti-mouse/human CD11b

  • PE/Cyanine7 anti-mouse/human CD11b

  • Alexa Fluor® 488 anti-mouse/human CD11b

  • Alexa Fluor® 647 anti-mouse/human CD11b

  • Alexa Fluor® 700 anti-mouse/human CD11b

  • Pacific Blue™ anti-mouse/human CD11b

  • APC/Cyanine7 anti-mouse/human CD11b

  • PerCP/Cyanine5.5 anti-mouse/human CD11b

  • PerCP anti-mouse/human CD11b

  • Brilliant Violet 421™ anti-mouse/human CD11b

  • Brilliant Violet 570™ anti-mouse/human CD11b

  • Brilliant Violet 605™ anti-mouse/human CD11b

  • Brilliant Violet 650™ anti-mouse/human CD11b

  • Brilliant Violet 711™ anti-mouse/human CD11b

  • Brilliant Violet 785™ anti-mouse/human CD11b

  • Brilliant Violet 510™ anti-mouse/human CD11b

  • Ultra-LEAF™ Purified anti-mouse/human CD11b

  • Purified anti-mouse/human CD11b (Maxpar® Ready)

  • Alexa Fluor® 594 anti-mouse/human CD11b

  • PE/Dazzle™ 594 anti-mouse/human CD11b

  • APC/Fire™ 750 anti-mouse/human CD11b

  • TotalSeq™-A0014 anti-mouse/human CD11b

  • Brilliant Violet 750™ anti-mouse/human CD11b

  • TotalSeq™-B0014 anti-mouse/human CD11b

  • TotalSeq™-C0014 anti-mouse/human CD11b

  • Spark NIR™ 685 anti-mouse/human CD11b

  • PE/Fire™ 640 anti-mouse/human CD11b

  • Spark YG™ 593 anti-mouse/human CD11b

  • Spark YG™ 570 anti-mouse/human CD11b

  • PE/Fire™ 810 anti-mouse/human CD11b

  • APC/Fire™ 810 anti-mouse/human CD11b Antibody

  • Spark Blue™ 550 anti-mouse/human CD11b

  • Spark UV™ 387 anti-mouse/human CD11b

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account