- JES3-12G8 (See other available formats)
- Other Names
- Interleukin-10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
- Rat IgG2a, κ
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- Product Citations
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IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-12G8 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-12G8 antibody can neutralize the bioactivity of natural or recombinant IL-10.Product Details
- Antibody Type
- Host Species
- COS - expressed, recombinant human IL-10
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection, ELISPOT Detection
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 500 to 2 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection1-4 or ELISPOT Detection5: The biotinylated JES3-12G8 antibody is useful as a detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the Ultra-LEAF™ purified JES3-9D7 antibody (Cat. Nos. 501427 & 501428) or purified JES3-19F1 antibody (Cat. No. 506802/506810) as capture antibody and recombinant human IL-10 (Cat. No. 571009) as the standard.
Additional reported applications (for the relevant formats) include: immunohistochemical staining6,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, neutralization, and immunocytochemistry.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.
- Application References
- Abrams, J., et al. 1992. Immunol. Rev. 127:5.
- Gotlieb, W., et al. 1992. Cytokine 4:385. (ELISA)
- Yssel, H., et al. 1992 J. Immunol. 149:2378. (ELISA)
- Burdin, N., et al. 1993. J. Exp. Med. 177:295. (ELISA)
- Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.
- Tazi, A., et al. 1999. J. Immunol.163:3511
- Andersson, J., et al. 1994. Immunology 83:16. (IHC)
- Product Citations
AB_315177 (BioLegend Cat. No. 501501)
AB_315178 (BioLegend Cat. No. 501502)
- Acid-labile cytokine; dimer; 35-40 kD (Mammalian)
- Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
- Cell Sources
- Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
- Cell Targets
- T cells, B cells, mast cells, macrophages
- IL-10R (CDw210)
- Cell Type
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. de Waal-Malefyt, R., et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard, M., et al. 1992. Immunol. Today. 13:198.
4. Quesniaux, V. 1992. Research Immunol. 143:385.
- Production inhibited by IL-4, IL-10
- Gene ID
- 3586 View all products for this Gene ID
- View information about IL-10 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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