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APC anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_APC_081908.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
  • G8dot8_APC_081908.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
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118213 25 µg 82€
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118214 100 µg 237€
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chen Q, et al. 2020. Cell Reports. 31(13):107828. PubMed
  2. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  3. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  4. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  5. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  6. Elkon R, et al. 2015. Nat Commun. 6: 8549. PubMed
  7. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
  8. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  9. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  10. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  11. Mevel R, et al. 2020. eLife. 9:00. PubMed
  12. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
  13. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  14. Buerger S, et al. 2015. J Immunol. 195: 4106 - 4116. PubMed
  15. Malhotra D, et al. 2016. Nat Immunol. 17:187-195. PubMed
  16. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  17. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  18. Ford MJ, et al. 2021. Cell Rep. 36:109677. PubMed
  19. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  20. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  21. Phung HT, et al. 2020. Immunohorizons. 0.25625. PubMed
  22. Chua CW, et al. 2018. Elife. 7. PubMed
  23. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  24. Semba T, et al. 2020. Cancers (Basel). 12:. PubMed
  25. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
  26. Willobee BA, et al. 2021. Mol Cancer Ther. 20:1246. PubMed
  27. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  28. Katara GK, et al. 2020. Mol Oncol. 0.729861111. PubMed
  29. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  30. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  31. Wang Y, et al. 2014. PLoS One. 9:113127. PubMed
  32. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  33. Rudnick JA, et al. 2021. Genes Dev. 35:963. PubMed
  34. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  35. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  36. Bar C, et al. 2020. Cell Reports. 28(1):257-266.e5.. PubMed
  37. Jiang M, et al. 2021. Developmental Cell. 56(11):1646-1660.e5. PubMed
  38. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  39. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  40. Sateriale A, et al. 2020. Cell Host & Microbe. 26(1):135-146.e5.. PubMed
  41. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  42. Ionkina AA, et al. 2021. Breast Cancer Res. 23:93. PubMed
  43. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  44. Hertzano R, et al. 2020. Laryngoscope. . PubMed
  45. Li J, et al. 2020. Development. :. PubMed
  46. Cerretelli G, et al. 2021. J Pathol. 255:464. PubMed
  47. Tang Z, et al. 2021. Stem Cell Res Ther. 12:397. PubMed
  48. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  49. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  50. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  51. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  52. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  53. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  54. Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed
  55. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  56. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  57. Caporarello N, et al. 2020. Aging Cell. 19:e13196. PubMed
  58. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  59. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  60. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  61. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  62. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  63. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  64. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  65. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  66. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  67. Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed
  68. Casey O, et al. 2012. PLoS One. 7:e41668. PubMed
  69. Tanimizu N, et al. 2014. J Biol Chem. 289:7859. PubMed
  70. Kasuga A, et al. 2020. Cancer Sci. . PubMed
  71. Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed
  72. Jadhav U, et al. 2019. Mol Cell. 74:542. PubMed
  73. Renz BW, et al. 2018. Cancer Cell. 33:75. PubMed
  74. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
  75. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  76. Chen Z, et al. 2020. Immunity. 54(1):151-163.e6. PubMed
  77. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
RRID
AB_1134105 (BioLegend Cat. No. 118213)
AB_1134102 (BioLegend Cat. No. 118214)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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