- MIH24 (See other available formats)
- Regulatory Status
- Other Names
- TRANCE (TNF-related activation-induced cytokine receptor), CD254, RANK-L (receptor activator of NF-κB ligand), OPGL (Osteoprotegerin ligand), SOFA, TNFSF-11
- Mouse IgG2b, κ
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- Product Citations
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Human CD254, also known as RANKL, TRANCE, and OPGL, is a type II transmembrane protein in the TNF superfamily that has two forms: a membrane-anchored protein and a secreted protein. Its extracellular domain is related to TRAIL, FasL, and TNF. CD254 is expressed on osteoblasts and activated T cells. It has been implicated in the regulation of the interactions between T cells and dendritic cells. It plays an important role in osteoblast differentiation and bone resorption. CD254 activates JNK and NF-κB and induces the expression of IL-1, IL-6, IL-12, and IL-15 in dendritic cells.Product Details
- Antibody Type
- Host Species
- RANKL-transfected L cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: ELISA and immunohistochemical staining of paraffin-embedded tissue sections2.
- Application References
- Kanamaru F, et al. 2004. Immunol. Lett. 94:239.
- Mueller A, et al. 2014. Arthritis Res. Ther. 16:R55. (IHC) PubMed
- Product Citations
AB_2564322 (BioLegend Cat. No. 347507)
AB_2564323 (BioLegend Cat. No. 347508)
- A TNF superfamily member.
Expressed on osteoblasts and activated T cells.
- Plays an important role in osteoblast differentiation and bone resorption, regulates the interactions between T cells and dendritic cells, and T cells and B cells.
- RANK, OPG.
- Cell Type
- T cells
- Biology Area
- Molecular Family
- CD Molecules, Cytokines/Chemokines
- Antigen References
1. Wiethe C, et al. 2003. J. Immunol. 171:4121
2. Page G, et al. 2005. Arthritis Rheum. 52:2307
3. Ji JD, et al. 2009. J. Immunol. 183:7223
- Gene ID
- 8600 View all products for this Gene ID
- View information about CD254 on UniProt.org
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