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Ultra-LEAF™ Purified anti-mouse Thrombopoietin (TPO) Antibody

Pricing & Availability
Clone
A15138F (See other available formats)
Regulatory Status
RUO
Other Names
Megakaryocyte Colony Stimulating Factor (MKCSF), Myeloproliferative leukemia virus oncogene ligand (Mpl-ligand), Megakaryocyte growth and development factor (MGDF), THPO
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A15138F_ULEAF_Thrombopoietin_TPO_Antibody_051617
Recombinant mouse TPO induces proliferation of human megakaryocytic leukemic MO7e cells in a dose dependent manner (black circles). Anti-mouse TPO monoclonal antibody (clone A15139F, purple circles) neutralizes the proliferation of MO7e cells induced by recombinant mouse TPO (1 ng/ml). The ND50 = 0.04 – 0.2 µg/ml.
  • A15138F_ULEAF_Thrombopoietin_TPO_Antibody_051617
    Recombinant mouse TPO induces proliferation of human megakaryocytic leukemic MO7e cells in a dose dependent manner (black circles). Anti-mouse TPO monoclonal antibody (clone A15139F, purple circles) neutralizes the proliferation of MO7e cells induced by recombinant mouse TPO (1 ng/ml). The ND50 = 0.04 – 0.2 µg/ml.
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696103 100 µg 216€
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696104 1 mg 572€
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Description

Mouse thrombopoietin (TPO) is a protein consisting of an N-terminus domain with homology to erythropoietin (EPO) and a C-terminus domain with multiple N-linked and O-linked glycosylation sites. TPO is a key regulator of megakaryocytopoiesis and thrombopoiesis. It signals through the c-mpl receptor and stimulates the proliferation and maturation of megakaryocytes, hence, TPO plays an important role in regulating the level of circulating platelets in vivo. It has also been shown that TPO is able to promote the survival, self-renewal, and expansion of hematopoietic stem cells and primitive multilineage progenitors. Besides its hematopoietic effects, TPO is expressed in the brain where it promotes the apoptosis of hypoxia sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse TPO
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing activity. The ND50 is 0.04 – 0.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
RRID
AB_2687033 (BioLegend Cat. No. 696103)
AB_2687034 (BioLegend Cat. No. 696104)

Antigen Details

Structure
Monomer
Distribution

TPO is produced by liver and kidney.

Function
TPO regulates megakaryocytopoiesis and thrombopoiesis.
Interaction
Megakaryocytes
Ligand/Receptor
TPO-R (MPL, CD110)
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Avanzi G, et al. 1988. Br. J. Haematol. 69:359. 
2. Deutsch VR and Tomer A. 2006. Br. J. Haematol. 134:453. 
3. Kaushansky K. 2005. J. Clin. Invest. 115:3339. 
4. Li J, et al. 1999. Br. J. Haematol. 106:345. 
5. Kato T, et al. 1997. Proc. Natl. Acad. Sci. 94:4669. 
6. Ehrenreich H, et al. 2005. Proc. Natl. Acad. Sci. 102:862.
7. Sangkhae V, et al. 2014. Blood 124:3956.

Gene ID
22018 View all products for this Gene ID
UniProt
View information about Thrombopoietin on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 0    Revision Date: 05/17/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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