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Purified anti-NRF2 Antibody

Pricing & Availability
Clone
W19086B (See other available formats)
Regulatory Status
RUO
Other Names
Erythroid 2 Like 2, NF-E2-Related Factor 2, HEBP1, IMDDHH
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19086B_PURE_NRF2_Antibody_1_041521
Whole cell lysates (15 µg) from untreated (-) or MG-132 treated (+) (10 µM, 10 hours) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of purified anti-NRF2 antibody (clone W19086B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405422) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W19086B_PURE_NRF2_Antibody_1_041521
    Whole cell lysates (15 µg) from untreated (-) or MG-132 treated (+) (10 µM, 10 hours) HeLa cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 µg/mL (1:500 dilution) of purified anti-NRF2 antibody (clone W19086B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405422) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W19086B_PURE_NRF2_Antibody_3_041521
    Untreated (panel A) or MG-132 treated (panel B) (10 µM, 10 hours) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 30 minutes. Cells were then intracellularly stained with a 1.0 µg/mL (1:500 dilution) of purified anti-NRF2 antibody (clone W19086B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG antibody (Cat. No. 405422) at 2.5 µg/mL. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • W19086B_PURE_NRF2_Antibody_4_041521.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells treated with MG-132 (10 µM, 10 hours) were immunoprecipitated overnight with 2.5 µg of purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-NRF2 antibody (clone W19086B). The resulting IP fractions and whole cell extract input (10%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with purified anti-NRF2 Antibody (clone W19086B). Lane M: Molecular weight marker.
  • W19086B_PURE_NRF2_Antibody_5_041521
    IHC staining of purified anti-NRF2 antibody (clone W19086B) on formalin-fixed paraffin-embedded glioblastoma tissues. Following antigen retrieval using sodium citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • W19086B_PURE_NRF2_Antibody_7_102821
    HeLa cells were incubated overnight with (filled histogram) or without (open histogram) the proteasome inhibitor MG132. Cells were fixed with True-Nuclear™ Fix buffer and permeabilized with True-Nuclear™ Perm Buffer. Cells were then stained with purified anti-NRF2 antibody (clone W19086B) followed by anti-rat IgG PE.
  • W19086B_PURE_NRF2_Antibody_WB_120922
    Whole cell lysates (15 μg) from untreated (-) or MG-132 treated (+) (10 μM, 10 hours) NIH/3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane and probed with 1.0 μg/mL (1:500 dilution) of purified anti-NRF2 antibody (clone W19086B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker
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939201 25 µg 109€
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939202 100 µg 268€
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Description

NRF2 is a transcription factor that plays a critical role in inducing expression of genes required for oxidative stress defense and stress balance. It does so by binding to antioxidant response elements (AREs), which are located upstream of target genes. NRF2 is degraded by ubiquitination of KEAP1 E3 ligase. In a clinical setting, NRF2 is frequently activated in many types of cancers; it drives metabolic adaptation and survival in ROS-rich tumor microenvironments.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human NRF2 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, IHC-P, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry, a concentration range of 5.0 - 20.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on 4% PFA-fixed HeLa cells and permeabilized with either methanol or Triton X-100. Both permeabilization methods were compatible with NRF2 staining.

Clone W19086B is validated to detect NRF2 in mouse NIH/3T3 cells using a Western blot on whole cell lysate.

Product Citations
  1. Gupta A, et al. 2022. JCI Insight. . PubMed
RRID
AB_2892502 (BioLegend Cat. No. 939201)
AB_2892502 (BioLegend Cat. No. 939202)

Antigen Details

Structure
NRF2 is a 605 amino acid protein with a predicted molecular weight of 67 kD.
Distribution

Ubiquitously expressed/Nucleus

Function
Transcription factor/Redox balance
Biology Area
Cell Biology, Mitochondrial Function, Transcription Factors
Antigen References
  1. M. da Costa R, et al. 2019. Front In Pharm. 10:3389.
  2. Shoemaker A. 2017. Sci Trans Med. 9:420.
  3. Robledinos-Antón N, et al. 2019. Hindawi. 10:1155.
  4. Cuadrado A, et al. 2019. Nat Rev Drug Discov. 18:295-317.  
Gene ID
4780 View all products for this Gene ID
UniProt
View information about NRF2 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All NRF2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-NRF2 W19086B WB,ICC,IP,IHC-P,ICFC
PE anti-NRF2 W19086B ICFC
Alexa Fluor® 647 anti-NRF2 W19086B ICFC
Go To Top Version: 3    Revision Date: 12/09/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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