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Purified anti-mouse CCL8 (MCP-2) Antibody

Pricing & Availability
Clone
A16070K (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemotactic protein 2, MCP-2, small inducible cytokine subfamily A, member 8 (SCYA8),Chemokine (C-C motif) ligand 8, CCL8, MCP2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
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  • A16070K_Pure_CCL8_Antibody_121218.png
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536902 500 µg 465€
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Description

CCL8, also known as MCP-2, is a CC chemokine of the monocyte chemoattractant protein (MCP) family that plays key roles in allergic and inflammatory responses. CCL8 (MCP-2) has structural and functional similarity to other family members MCP-1 and MCP-3, and is often co-expressed with these chemokines in mononuclear cells. CCL8 is also constitutively expressed in the skin and lymph nodes of mice.

Mouse CCL8 induces a chemotactic migration of a population of differentiated Th2 cells enriched in IL-5 and IL-25 receptors by binding to the CCR8 receptor. Recent studies have established CCL8 as a critical ligand for driving CCR8-dependent Th2 cell trafficking into the skin, which in conjunction with locally recruited eosinophils drives chronic cutaneous allergic inflammation. CCL8 expression by suprabasal buldge cells has also been experimentally shown to negatively regulate the population of mouse hair follicles by Langerhans cells, further underscoring this chemokines central contributions to maintaining cutaneous immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.5 - 2 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CCL8 (MCP-2) recombinant protein ranging from 15.6 to 1,000 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified A16070K antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5352 antibody (Cat. No. 535203) as the detection antibody and Recombinant Mouse CCL8 (MCP-2) (ELISA Std.) (Cat. No. 581709)

RRID
AB_2783344 (BioLegend Cat. No. 536902)

Antigen Details

Structure
Chemokine
Distribution

Fibroblast, endothelial cells, monocytes, macrophages, and skeletal muscle cells.

Function
CCL8 chemoattracts monocytes, T cells, NK cells, mast cells, eosinophils, and basophils. CCL8 is induced by IL-1β, IFNβ, IFNγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
Interaction
Monocytes, T cells, NK cells, mast cells, eosinophils, and basophils.
Ligand/Receptor
CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195)
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Monocytes
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Van Damme J, et al. 1992. J. Exp. Med. 176:59.
  2. Proost P, et al. 1998. J. Immunol. 160:4034.
  3. Dean RA, et al. 2008. Blood 112: 3455.
  4. Hori T, et al. 2008. Blood 111:4403.
  5. Rom S, et al. 2010. FASEB J. 24:2292.
  6. Struyf S, et al. 2009. Eur. J. Immunol. 39:843.
  7. Islam SA, et al. 2011. Nat. Immunol. 12:167.
Gene ID
20307 View all products for this Gene ID
UniProt
View information about CCL8 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/10/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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  • Purified anti-mouse CCL8 (MCP-2)

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