LEGEND MAX™ Total TGF-β1 ELISA Kit

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Other Names
Total TGF-β1 Pre-coated ELISA Kit
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Product Citations
publications
TGF-b1_020711
  • TGF-b1_020711
Cat # Size Price Quantity Save
436707 1 Pre-coated Plate 340€
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Description

Transforming growth factor beta 1 (TGF-β1) is a member of the transforming growth factor beta superfamily of cytokines. TGF- β1 precursor contains 390 amino acids with an N-terminal signal peptide of 29 amino acids required for secretion from a cell, a 249 amino acids pro-region ( latency associated peptide or LAP), and a 112 amino acids C-terminal region that becomes the active TGF- β1 upon activation.

Both LAP and TGF- β1 exist as homodimers in circulation, but the disulfide linked homodimers of LAP and TGF- β1 remain non-covalently associated, forming the small latent TGFβ1 complex (SLC, 100 kD).  The large latent TGF- β1 Complex (LLC, 235 – 260 kD) contains a third component, the latent TGF- ß binding protein (LTBP), which is linked to LAP by a single disulfide bond.  The LTBP does not confer latency, but for efficient secretion of the complex to extracellular sites.  Free active TGF- β1 can be released (activated) by many factors including enzymes and low or high pH.

TGF- β1 is nearly 100% conserved across mammalian species. It has diverse biological functions in multiple cellular processes such as regulating proliferation and differentiation of various cell types. TGF- β1 is also an important immunoregulatory cytokine, which is involved in the maintenance of self-tolerance, Th17 differentiation, and T cell homeostasis etc.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-TGF-β1 Pre-coated 96-well Strip Microplate
  • TGF-β1 Dectection Antibody
  • TGF-β1 Standard
  • Avidin-HRP D
  • Assay Buffer C
  • Wash Buffer (20X)
  • Substrate Solution F
  • Sample Diluent
  • Acidification Solution
  • Neutralization Solution
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human, Mouse, Rat, Non-human Primates, Bovine, Porcine, Ovine, Rabbit.
Application

ELISA

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References
  1. Park GB, et al. 2014. Invest Opthalmol Vis Sci. 55:1770. PubMed
  2. Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
Product Citations
  1. Park G, et al. 2014. Invest Ophthalmol Vis Sci . 55:1770. PubMed
  2. Johannesson B, et al. 2014. Dis Model Mech. 7:977. PubMed
  3. O’Conor C, et al. 2016. Sci Rep. 6:29053. PubMed
  4. Chen C, et al. 2016. J Lipid Res. 57: 1435 - 1446. PubMed
  5. Choi J, et al. 2017. Sci Rep. 7:43349. PubMed
  6. Derakhshandeh R, et al. 2018. Oncotarget. 9:11126. PubMed
  7. Orgaz JL, et al. 2020. Cancer Cell. 37:85. PubMed
Sensitivity
3.5 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Many cell types, highly expressed on activated Tregs and platelets
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
21803 View all products for this Gene ID
UniProt
View information about TGF-beta1 on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

Go To Top Version: 7    Revision Date: 01/19/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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