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APC anti-human CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
H4A3 (See other available formats)
Regulatory Status
RUO
Workshop
P PR-63; BP 473; P P008
Other Names
Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
H4A3_APC_110308.jpg
Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) APC (pink histogram) or mouse IgG1, κ APC (blue histogram).
  • H4A3_APC_110308.jpg
    Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) APC (pink histogram) or mouse IgG1, κ APC (blue histogram).
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328619 25 tests 120€
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328620 100 tests 252€
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human adult adherent peripheral blood cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References
  1. Misse D, et al. 1999. Blood 93:2454.
  2. Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
  3. Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
  4. Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
  5. Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
  6. De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
  7. Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
  8. Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
  9. Salio M, et al. 2013 PNAS. 110:4753. PubMed
Product Citations
  1. Fowler DW, et al. 2017. Cancer Immunol Immunother. 66:1205. PubMed
  2. Buijsers B, et al. 2022. Front Immunol. 12:759570. PubMed
  3. Ali A, et al. 2020. J Infect Dis. 222:853. PubMed
  4. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  5. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
  6. Yan LM, et al. 2020. MBio. 11:. PubMed
  7. Diab M, et al. 2020. Viruses. :12. PubMed
  8. Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
  9. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  10. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  11. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  12. Gujar S, et al. 2010. Mol Cancer Ther. 2.405555556. PubMed
  13. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  14. Sahillioglu AC, et al. 2021. Cancer Immunol Res. 9:999. PubMed
  15. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  16. Vinton C, et al. 2017. J Immunol. 10.4049/jimmunol.1700136. PubMed
  17. Guo M, et al. 2021. Front Oncol. 11:632540. PubMed
  18. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  19. Harper JL, et al. 2020. Nat Med. 519:26. PubMed
  20. Ding R, et al. 2021. Theranostics. 4957:11. PubMed
  21. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  22. Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
  23. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  24. Guo S, et al. 2021. Stem Cells Int. 2021:9989790. PubMed
  25. Davis Z, et al. 2016. PLoS Pathog. 12: 1005421. PubMed
  26. Han F, et al. 2022. Cent Eur J Immunol. 46:427. PubMed
  27. Georg P, et al. 2022. Cell. 185:493. PubMed
  28. López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed
  29. Elias S, et al. 2020. Haematologica. . PubMed
  30. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  31. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  32. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  33. Frasca D, et al. 2018. PLoS One. 13:e0197472. PubMed
  34. Iraqi M, et al. 2020. Front Cell Infect Microbiol. 10:48. PubMed
  35. Rambaldi B, et al. 2022. Haematologica. 107:2617. PubMed
  36. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  37. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  38. Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
  39. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
  40. Gruijs M, et al. 2021. Int J Mol Sci. 22:. PubMed
  41. Dao TN, et al. 2020. Cancers (Basel). 12:00. PubMed
RRID
AB_1279057 (BioLegend Cat. No. 328619)
AB_1279055 (BioLegend Cat. No. 328620)

Antigen Details

Structure
LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution

Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes

Function
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor
Galaptin
Cell Type
Endothelial cells, Epithelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.

Gene ID
3916 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10/22/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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