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Ultra-LEAF™ Purified anti-human CCL8 (MPC-2) Antibody

Pricing & Availability
Clone
A15150G (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemotactic protein 2 (MCP-2), C-C Motif Chemokine Ligand 8
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A15150G_ULEAF_CCL8_Antibody_1_062320_updated.png
Recombinant human CCL8 (Cat. No. 581606) ( black circles) induces the chemotaxis of human THP-1 cells. Anti-human CCL8 antibody (clone W15150G) (purple squares) neutralizes the chemotaxis of recombinant human CCL8 at 0.2 ng/mL in human THP-1 cells in a dose-dependent manner while the Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400264) (grey triangles) does not have the effect. ND50 range: 0.4-4 µg/mL.
  • A15150G_ULEAF_CCL8_Antibody_1_062320_updated.png
    Recombinant human CCL8 (Cat. No. 581606) ( black circles) induces the chemotaxis of human THP-1 cells. Anti-human CCL8 antibody (clone W15150G) (purple squares) neutralizes the chemotaxis of recombinant human CCL8 at 0.2 ng/mL in human THP-1 cells in a dose-dependent manner while the Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400264) (grey triangles) does not have the effect. ND50 range: 0.4-4 µg/mL.
Cat # Size Price Save
933203 100 µg ¥44,820
933204 1 mg ¥118,690
Description

CCL8, also known as MCP-2, is a beta chemokine initially isolated from human MG-63 osteosarcoma cells stimulated with IL-1β. CCL8 (MCP-2) has structural and functional similarity with MCP-1 and MCP-3, and it is often co-induced with those chemokines in mononuclear cells. IFN-γ is a strong inducer of CCL8 in monocytes and fibroblasts. CCL8 can be processed and secreted as a truncated chemokine CCL8 (6-75). This isoform does not chemoattract monocytes, but it binds to the CCR2 receptor and induces its internalization; as a result, CCL8 inhibits MCP-1 (CCL2) and MCP-2 (CCL8) ERK signaling and antagonizes the chemotactic activity of several CCR2 ligands (MCP-1/CCL2, MCP-2/CCL8, MCP-3/CCL7). CCL8 is highly expressed in the skin, and it serves as an agonist for the chemokine receptor CCR8, but not for CCR2. Therefore, it has been speculated that the CCL8-CCR8 interaction is a crucial regulator of Th2 cell homing, which drives IL-5-mediated chronic allergic inflammation. In addition, CCL8 binds CCR5 receptor with high affinity and inhibits the replication of human immunodeficiency virus type 1. CCL8 is highly expressed in different human diseases, and it is a potential biomarker for the diagnosis of graft-versus-host diseases and tuberculosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CCL8
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CCL8 (0.2 µg/mL) on human THP-1 cells. ND50 range: 0.4 - 4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2861115 (BioLegend Cat. No. 933203)
AB_2861116 (BioLegend Cat. No. 933204)

Antigen Details

Structure
Chemokine
Distribution

Fibroblast, endothelial cells, monocytes, and macrophages

Function
CCL8 chemoattracts monocytes, T cells, NK cells, mast cells, eosinophils, and basophils. CCL8 is induced by IL-1β, IFNβ, INFγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
Interaction
Monocytes, T cells, NK cells, mast cells, eosinophils, and basophils
Ligand/Receptor
CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195)
Cell Type
Basophils, Eosinophils, Mast cells, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Van Damme J, et al. 1992. J Exp Med. 176:59-65.
  2. Proost P, et al. 1998. J Immunol. 160:4034-41.
  3. Dean RA, et al. 2008. Blood. 112: 3455-64.
  4. Hori T, et al. 2008. Blood. 111:4403-12.
  5. Rom S, et al. 2010. FASEB J. 24:2292-300.
  6. Struyf S, et al. 2009. Eur J Immunol. 39:843-57.
  7. Islam SA, et al. 2011. Nat Immunol. 12:167-77.
Gene ID
6355 View all products for this Gene ID
UniProt
View information about CCL8 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/09/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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