Recombinant Human BMP-9 (carrier-free)

Pricing & Availability
Other Names
Bone morphogenetic protein 9 (BMP9), Growth/differentiation factor 2 (GDF2)
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Product Citations
publications
BMP-9_Human_RECOM_BA_062714.jpg
Alkaline phosphatase induced by human BMP-9 in ATDC5 cells.
  • BMP-9_Human_RECOM_BA_062714.jpg
    Alkaline phosphatase induced by human BMP-9 in ATDC5 cells.
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Cat # Size Price Save
553102 10 µg ¥33,300
553104 25 µg ¥53,100
Description

BMP-9 was initially isolated from fetal mouse liver cDNA libraries. The mouse and human proteins share 84% identity. They are part of the bone morphogenetic protein (BMP) family of proteins, which are members of the TGF-β superfamily and play a key role in skeletal development, bone formation, and stem cell differentiation. BMP-9 is one of the most osteogenic factors of the family. It is synthetized by hepatocytes and intrahepatic biliary epithelial cells. It circulates in plasma in an unprocessed, inactive form, that can be further activated by furin as well as a mature and fully active complex form bound to its prodomain. BMPs signal through heterotetrameric complex receptors, which include four type I receptors (ALK-1, ALK-2, ALK-3, and ALK-6) and three type II receptors (BMPRII, ActRIIA, and ActRIIB). BMP-9 and BMP-10 bind to ALK-1 (mainly expressed in endothelial cells). BMP-9 inhibits the proliferation of endothelial cells induced by basic FGF and VEGF-stimulated angiogenesis by binding to ALK-1. BMP-9 also binds to ALK-2 in primary myeloma cells, and this binding can be inhibited by membrane-bound or soluble endoglin (an accessory receptor for the TGF-β family). BMP-9 activity is inhibited by crossveinless-2 (CV2), a member of the chordin family and an extracellular regulator. BMP-9 induces CV2 expression in endothelial cells and in a negative feedback loop inhibits its own activity by the binding of CV2 to BMP-9, which consequently inhibits its binding to ALK-1. BMP-9 and -10 possess tumor suppressing activity in breast and prostate cancer; BMP-9 also induces apoptosis in primary myeloma cells. In contrast, autocrine BMP-9 signaling induces proliferation of ovarian and liver cancer cells.

Product Details
Technical data sheet

Product Details

Source
Human BMP-9, amino acids (Ser320 - Arg429) (Accession# NM_016204), was expressed in CHO cells.
Molecular Mass
The 110 amino acid recombinant protein has a predicted molecular mass of approximately 12 kD. The DTT-reduced and non-reduced protein migrate at approximately 14 kD and 24 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 0.1% TFA, 30% ACN.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.4 - 2 ng/ml, corresponding to a specific activity of 0.5 - 2.5 x 106 units/mg, as determined by induction of alkaline phosphatase in ATDC5 cells in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Wang W, et al. 2019. Proc Natl Acad Sci U S A. 116:15570. PubMed
  2. Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed

Antigen Details

Structure
Homodimer
Distribution
Hepatocytes, non parenchimal cells of the liver, endothelial, stellate cells, osteosarcoma cells.
Function
Induces osteogenesis, chondrogenesis, and differentiation of cholinergic neurons, regulates glucose metabolism.
Interaction
Endothelial cells, microvascular endothelial cells, prostate cancer cells, ovarian cancer cells, osteosarcoma cells, primary myeloma cells.
Ligand/Receptor
Activin receptor-like kinase- ALK-1 and ALK-5 in endothelial cells; ALK-1 and BMPR-II in endothelial cells; ALK-1 and ALK-2 in mesenchymal stem cells; ALK-2 and BMPR-II in myoblasts; ALK-1 and endoglin in glioblastoma cells. Endoglin (CD105).
Bioactivity
Induction of alkaline phosphatase in ATDC5 cells.
Cell Type
Neural Stem Cells, Mesenchymal Stem Cells, Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Miller AF, et al. 2000. J. Bio. Chem. 275:17937.
2. Chen C, et al. 2003. Nat. Biotechnol. 21:294.
3. Scharpfenecker M, et al. 2007. J. Cell Sciences 120:964.
4. Luo J, et al. 2010. J. Biol. Chem. 285:29588.
5. Bidart M, et al. 2012. Cell Mol. Life Sci. 69:313-24.
6. Yao Y, et al. 2012. Blood 119:5037.
7. Olsen OE, et al. 2014. Blood Cancer J. 4:e196.
8. Herrera B, et al. 2014. Int. J. Mol. Sci. 15:5199.

Gene ID
2658 View all products for this Gene ID
UniProt
View information about BMP-9 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 06/30/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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