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Purified anti-IL-18 Antibody

Pricing & Availability
Clone
W18160A (See other available formats)
Regulatory Status
RUO
Other Names
IL18, IL-18, Interleukin 18, Interleukin-18, IL-1 Gamma, Interleukin-1 Gamma, IFN-Gamma-Inducing Factor
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W18160A_PURE_IL-18_Antibody_1_111119
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of purified anti-IL-18 antibody (clone W18160A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18160A_PURE_IL-18_Antibody_1_111119
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.1 µg/mL of purified anti-IL-18 antibody (clone W18160A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
  • W18160A_PURE_IL-18_Antibody_3_111119
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes at -20°C, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 1 µg/mL of either Purified Rat IgG2a, κ Isotype Ctrl Antibody (Cat. No. 400502, panel A) or purified anti-IL-18 antibody (clone W18160A) (panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG Antibody (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
Cat # Size Price Save
934801 25 µg ¥22,220
934802 100 µg ¥55,660
Description

Interleukin 18 (IL-18) is a proinflammatory cytokine that participates in both innate and acquired immunity. IL-18 is a part of the IL-1 superfamily that activates T-helper 1 (Th1) and natural killer (NK) cells by inducing IFN-γ production. IL-18 is produced in its inactive precursor form, pro-IL-18 (24 kD), with no signal peptide, accumulating in the cytoplasm. Pro-IL-18 is then processed intracellularly by CASP1 into the mature active form of IL-18 (18 kD) which is secreted extracellularly. Mature IL-18 binds to an inducible receptor, IL-18Rα, and then recruits IL-18Rβ which initiates an immune reaction through TLR/IL-1R-like signaling by recruiting MyD88 and the NF-κB pathway. IL-18 is associated with Adult-onset Still's disease and pleural tuberculosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human IL-18 protein of approximately 60 amino acids
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone recognizes human IL-18.

For ICC testing, HeLa cells were fixed in 4% PFA and permeabilized with either Triton X-100 or methanol; both methods produced strong staining at 1 mg/mL.

RRID
AB_2820219 (BioLegend Cat. No. 934801)
AB_2820220 (BioLegend Cat. No. 934802)

Antigen Details

Structure
IL-18 is a 193 amino acid protein with a predicted molecular weight of 22 kD.
Distribution

IL-18 is secreted, also localized to the nucleus and cytosol.

Function
Proinflammatory cytokine
Ligand/Receptor
Binds inducible receptor IL-18Rα, and then recruits IL-18Rβ
Cell Type
NK cells, T cells
Biology Area
Adaptive Immunity, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Adachi O, et al. 1998. Cell Press. 9:1:143.
  2. Akira S, et al. 2000. Curr. Op. Imm. 12:1:59.
  3. Van de Veerdonk, et al. 2011. Trends in Imm. 32:3:110.
  4. Canna S, et al. 2017. Jour. of All. and Clin. Imm. 139:5:1698.
  5. Muhl H, et al. 2019. Cell Sig. 63:109388.
Gene ID
3606 View all products for this Gene ID
UniProt
View information about IL-18 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/11/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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