Loading...

Purified anti-human CD206 (MMR) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
15-2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
15-2_Purified_CD206_Cytof_1_060314.jpg
Human PBMCs were incubated for 3 days in media alone (top) or with GM-CSF (bottom). Cells were then stained with 159Tb-anti-CD11c (Bu15) and 168Er-anti-CD206/MMR (15-2). CD33+ Monocyte cells are displayed in the analysis. Data provided by DVS Sciences.
  • 15-2_Purified_CD206_Cytof_1_060314.jpg
    Human PBMCs were incubated for 3 days in media alone (top) or with GM-CSF (bottom). Cells were then stained with 159Tb-anti-CD11c (Bu15) and 168Er-anti-CD206/MMR (15-2). CD33+ Monocyte cells are displayed in the analysis. Data provided by DVS Sciences.
  • 15-2_Purified_CD206_Cytof_2_060314.jpg
Compare all formats
Cat # Size Price Save
321127 100 µg ¥31,020
Description

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human mannose receptor
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block)
  2. Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block)
  3. Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations
  1. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  2. Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
RRID
AB_2563729 (BioLegend Cat. No. 321127)

Antigen Details

Structure
Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution

Macrophages, dendritic cells, hepatic and lymphatic endothelial cells

Function
Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor
Mannose, fucose, N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303
2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469.
4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934.
5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.

Gene ID
4360 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD206 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD206 (MMR) 15-2 FC,WB,ICC,IHC-F
FITC anti-human CD206 (MMR) 15-2 FC
PE anti-human CD206 (MMR) 15-2 FC
PE/Cyanine5 anti-human CD206 (MMR) 15-2 FC
APC anti-human CD206 (MMR) 15-2 FC
Alexa Fluor® 488 anti-human CD206 (MMR) 15-2 FC
Alexa Fluor® 647 anti-human CD206 (MMR) 15-2 FC
Biotin anti-human CD206 (MMR) 15-2 FC
APC/Cyanine7 anti-human CD206 (MMR) 15-2 FC
PerCP/Cyanine5.5 anti-human CD206 (MMR) 15-2 FC
PE/Cyanine7 anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 421™ anti-human CD206 (MMR) 15-2 FC
Purified anti-human CD206 (MMR) (Maxpar® Ready) 15-2 FC,CyTOF®
Alexa Fluor® 700 anti-human CD206 (MMR) 15-2 FC
PE/Dazzle™ 594 anti-human CD206 (MMR) 15-2 FC
APC/Fire™ 750 anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 711™ anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 510™ anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 605™ anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 785™ anti-human CD206 (MMR) 15-2 FC
TotalSeq™-A0205 anti-human CD206 (MMR) 15-2 PG
TotalSeq™-B0205 anti-human CD206 (MMR) 15-2 PG
TotalSeq™-C0205 anti-human CD206 (MMR) 15-2 PG
Ultra-LEAF™ Purified anti-human CD206 (MMR) 15-2 FC,WB,ICC,IHC-F
Pacific Blue™ anti-human CD206 (MMR) 15-2 FC
PE/Fire™ 700 anti-human CD206 (MMR) 15-2 FC
TotalSeq™-D0205 anti-human CD206 (MMR) 15-2 PG
Spark NIR™ 685 anti-human CD206 (MMR) 15-2 FC
Spark Red™ 718 anti-human CD206 (MMR) (Flexi-Fluor™) 15-2 FC
Go To Top Version: 2    Revision Date: 07/22/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account