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PE anti-mouse CD25 Antibody

Pricing & Availability
Clone
3C7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG2b, κ
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Product Citations
publications
3C7_PE_080207
Con A-activated (3days) C57BL/6 mouse splenocytes stained with 3C7 PE
  • 3C7_PE_080207
    Con A-activated (3days) C57BL/6 mouse splenocytes stained with 3C7 PE
Compare all formats See PE spectral data
Cat # Size Price Save
101903 25 µg ¥15,310
101904 100 µg ¥37,920
Description

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
  2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Lua Y, et al. 2012. Mol Immunol. 52:229. PubMed
  3. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  4. Benhamou D, et al. 2018. Front Immunol. 2.260416667. PubMed
  5. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  6. Sehnert B, et al. 2022. Front Immunol. 13:921488. PubMed
  7. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  8. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  9. Lian G et al. 2018. eLife. 7 pii: e36158. PubMed
  10. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  11. Roy S, et al. 2020. Sci Rep. 10:10992. PubMed
  12. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  13. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  14. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  15. An J, et al. 2022. iScience. 25:103570. PubMed
  16. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  17. Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed
  18. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  19. Sidwell T, et al. 2020. Nat Commun. 0.633333333. PubMed
  20. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  21. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  22. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  23. Bergin SM, et al. 2021. Brain Behav Immun. 95:477. PubMed
  24. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  25. Toyama S, et al. 2021. Int J Mol Sci. 22:. PubMed
  26. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  27. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  28. Xu X, et al. 2018. J Dermatol Sci. 91:134. PubMed
  29. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  30. Kyburz A, et al. 2017. Clin Exp Allergy. 47:1331. PubMed
  31. Toro Acevedo CA, et al. 2017. Front Microbiol. 8:2188. PubMed
  32. Zhang X, et al. 2021. Front Pharmacol. 12:629513. PubMed
  33. Niderla-Bielinska J, et al. 2021. Int J Mol Sci. :22. PubMed
  34. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  35. Haque A, et al. 2020. Exp Neurol. 330:113315. PubMed
  36. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  37. Godwin MS, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 320:L393. PubMed
  38. Montero-Barrera D, et al. 2015. BioMed Res Int. 2015 615865. PubMed
  39. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  40. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  41. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  42. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  43. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  44. Terlizzi M, et al. 2021. Cell Physiol Biochem. 55:539. PubMed
  45. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_312846 (BioLegend Cat. No. 101903)
AB_312847 (BioLegend Cat. No. 101904)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor, signal transduction
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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