PE anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
EH12.2H7 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
EH12dot2H7_PE_022508
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE (filled histogram) or mouse IgG1, κ PE (open histogram).
  • EH12dot2H7_PE_022508
    PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE (filled histogram) or mouse IgG1, κ PE (open histogram).
  • EH12dot2H7_PE_072209.jpg
    Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE and CD3 (clone UCHT1) PerCP/Cy5.5.
See PE spectral data
Cat # Size Price Save
329905 25 tests ¥22,000
329906 100 tests ¥48,000
Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical data sheet

Product Details

Reactivity
Human, African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The CD279 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3 and immunohistochemical staining of paraformaldehyde fixed frozen sections13. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
  3. Velu V, et al. 2007. J. Virol. 81:5819. (FA)
  4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
  5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
  6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
  7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
  8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
  9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
  10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
  11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
  12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
  13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Zhu C, et al. 2012. J Clin Endocrinol Metab. 97:943. PubMed
  2. Raghuraman S, et al. 2012. J Infect Dis. 205:763. PubMed
  3. Suzuki M, et al. 2012. J Immunol. 189:2118. PubMed
  4. Xu Y, et al. 2013. J Virol. 87:3760. PubMed
  5. Xu H, et al. 2013. J Leukoc Biol. 93:943. PubMed
  6. Fujita T, et al. 2014. J Immunol. 193:5576. PubMed
  7. Schumann K, et al. 2015. Proc Natl Acad Sci U S A. 112: 10437-10442. PubMed
  8. Colineau L, et al. 2015. PLoS One. 10: e0140978. PubMed
  9. Pombo C, et al. 2015. J Infect Dis. 12: 1376-1386. PubMed
  10. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  11. Nduom E, et al. 2016. Neuro Oncology. 18: 195 - 205. PubMed
  12. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  13. Richter M, et al. 2016. Mol Ther Methods Clin Dev. 5:16013. PubMed
  14. Beyer M, et al. 2016. Nat Immunol. 17:593-603. PubMed
  15. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  16. Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed
  17. Diao B, et al. 2020. Front Immunol. 1.032638889. PubMed
  18. Zhang C, et al. 2020. Front Oncol. 0.944444444. PubMed
  19. Li N, et al. 2020. Oncoimmunology. 9:1824643. PubMed
  20. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  21. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  22. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  23. Kamiya T, et al. 2018. Blood Adv. 2:517. PubMed
  24. Capuano C, et al. 2018. Front Immunol. 9:1031. PubMed
  25. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  26. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  27. Huang L, et al. 2018. Mol Med Rep. 18:77. PubMed
  28. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  29. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  30. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  31. Cortés–Rubio CN, et al. 2019. Clin Epigenetics. 11:134. PubMed
  32. Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed
  33. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  34. Pauthner M et al. 2017. Immunity. 46(6):1073-1088 . PubMed
  35. Wang B, et al. 2018. Mol Ther Nucleic Acids. 0.548611111. PubMed
  36. Li B, et al. 2019. Oncogenesis. 8:17. PubMed
  37. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  38. Wang C, et al. 2020. Oncologist. 25:382. PubMed
  39. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  40. Matsuyama H, et al. 2019. Sci Rep. 9:13181. PubMed
  41. Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed
  42. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  43. Chang W, et al. 2008. J Immunol. 181:6707. PubMed
  44. Vojnov L, et al. 2010. J Virol. 84:753. PubMed
  45. Radziewicz H, et al. 2010. J Immunol. 184:2410. PubMed
RRID
AB_940481 (BioLegend Cat. No. 329905)
AB_940483 (BioLegend Cat. No. 329906)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Ligand/Receptor
B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01/22/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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