- Other Names
- Multiplex assay, flow cytometry, Flex set, CBA, chemokine, chemotactic cytokine, immune surveillance, immune response, proinflammatory, CCL24 (Eotaxin-2), CCL8 (MCP-2), CXCL11 (ITAC), CX3CL1 (Fractalkine), CCL7 (MCP-3), XCL1 (Lymphotactin), CXCL2 (MIP-2), CCL9 (MIP-1γ)
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|741069||100 tests (3.5 mL)||¥500|
The LEGENDplex™ Mouse Proinflammatory Chemokine Panel 2 Detection Antibodies contains 8 anti-mouse antibodies against Eotaxin-2, MCP-2, ITAC, Fractalkine, MCP-3, Lymphotactin, MIP-2 and MIP-1γ. This Detection cocktail is intended for use with the following in Mix and Match assay reagents:
741071 LEGENDplex™ Mouse CCL24 (Eotaxin-2) Capture Bead A4, 13X
741072 LEGENDplex™ Mouse CCL8 (MCP-2) Capture Bead A5, 13X
741073 LEGENDplex™ Mouse CXCL11 (ITAC) Capture Bead A6, 13X
741074 LEGENDplex™ Mouse CX3CL1 (Fractalkine) Capture Bead A10, 13X
741075 LEGENDplex™ Mouse CCL7 (MCP-3) Capture Bead B2, 13X
741076 LEGENDplex™ Mouse XCL1 (Lymphotactin) Capture Bead B4, 13X
741077 LEGENDplex™ Mouse CXCL2 (MIP-2) Capture Bead B6, 13X
741079 LEGENDplex™ Mouse CCL9 (MIP-1γ) Capture Bead B9, 13X
741070 LEGENDplex™ Mouse Proinflammatory Chemokine Panel 2 Standard
741145 LEGENDplex™ Buffer Set O
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-bottom Plate
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are valid for 6 months from the date of receipt.