- Other Names
- Multiplex assay, Flex set, CBA, chemotactic cytokine, CXCL8 (IL-8), CXCL10 (IP-10), CCL11 (Eotaxin), CCL17 (TARC), CCL5 (RANTES), CCL3 (MIP-1α), CXCL9 (MIG), CCL20 (MIP-3α), CXCL1 (GROα), CXCL11 (I-TAC), CCL4 (MIP-1β), MCP-1
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- publications
| Cat # | Size | Price | Save |
|---|---|---|---|
| 740985 | 100 tests | ¥3,000 |
Chemotactic cytokines or chemokines play pivotal roles in various processes such as immune surveillance, organ development, angiogenesis, and immune responses. Expression profiling of chemokines, especially those involved in inflammation and immune disorders, is important in achieving a deeper understanding of disease states.
The Human Proinflammatory Chemokine Panel 1 is a multiplex bead-based assay panel, using fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 human chemokines, including MCP-1 (CCL2), RANTES (CCL5), IP-10 (CXCL10), Eotaxin (CCL11), TARC (CCL17), MIP-1α (CCL3), MIP-1β (CCL4), MIG (CXCL9), MIP-3α (CCL20), ENA-78 (CXCL5), GROα (CXCL1), I-TAC (CXCL11) and IL-8 (CXCL8). This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples.
The LEGENDplex™ Human Proinflammatory Chemokine Panel 1 (13-plex) is designed to allow flexible customization within the panel. The table below shows the panel configuration and sample dilution requirement.
|
Cat # |
Plex Size |
Targets |
Sample Type |
Dilution Factor |
|
740984, 740985 |
13-plex |
CCL2, CCL5, CXCL10, CCL11, CCL17, CCL3, CCL4, CXCL9, CCL20, CXCL5, CXCL1, CXCL11, CXCL8 |
Tissue Culture |
Varies |
|
741080, 741081 |
12-plex |
CCL2, CXCL10, CCL11, CCL17, CCL3, CCL4, CXCL9, CCL20, CXCL5, CXCL1, CXCL11, CXCL8 |
Tissue Culture |
Varies |
|
Serum/Plasma |
2X |
|||
|
741082, 741083 |
1-plex |
CCL5 |
Tissue Culture |
Varies |
|
Serum Plasma |
50x |
Please visit www.biolegend.com/legendplex for more information on how to mix and match within the panel.
Kit Contents
- Kit Contents
-
- Setup Beads 1: FITC Beads (1 mL)
- Setup Beads 2: PE Beads (1 mL)
- Setup Beads 3: Raw Beads (2 mL)
- Human Proinflammatory Chemokine Panel 1 (12-plex) Pre-mixed Beads (3.5 mL)
- Human CCL5 (RANTES) Capture Bead A10, 13X (270 µL)
- HU Proinflam. Chemokine Panel 1 Detection Antibodies (3.5 mL)
- HU Proinflam. Chemokine Panel 1 Standard Cocktail, Lyophilized (1 vial)
- LEGENDplex™ SA-PE (3.5 mL)
- LEGENDplex™ Matrix B, Lyophilized (1 vial)
- LEGENDplex™ Assay Buffer (25 mL)
- LEGENDplex™ Wash Buffer, 20X (25 mL)
- Plate Sealers (4 sheets)
- V-bottom Plate
Product Details
- Reactivity
- Human
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Materials Not Included
-
- Flow Cytometer
- Pipettes and Tips
- Reagent Reservoirs for Multichannel Pipettes
- Polypropylene Microfuge Tubes
- Vortex Mixer
- Sonicator
- Aluminum Foil
- Absorbent Pads or Paper Towels
- Plate Shaker
- Tabletop Centrifuges
- A Vacuum Filtration Unit and a Vacuum Source (if using filter plates)
- Centrifuge with a swinging bucket adaptor for microtiter plates (if using V-bottom plates)
- 1.1 mL polypropylene micro FACS tubes, in 96-tube rack
Antigen Details
- Gene ID
- 3576 View all products for this Gene ID 3627 View all products for this Gene ID 6356 View all products for this Gene ID 6361 View all products for this Gene ID 6352 View all products for this Gene ID 6348 View all products for this Gene ID 4283 View all products for this Gene ID 6364 View all products for this Gene ID 2919 View all products for this Gene ID 6351 View all products for this Gene ID 6373 View all products for this Gene ID 6347 View all products for this Gene ID 6374 View all products for this Gene ID
- UniProt
- View information about CXCL8, CXCL10, CCL11, CCL17, CCL3, CCL5, CXCL9, CCL20, CXCL1, CXCL11, CCL4, MCP-1, CXCL5 on UniProt.org
Related FAQs
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
-
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- If I don't have a vacuum, how do I remove the liquid from my plate?
-
Do NOT invert the plate to dump the liquid. The beads will not adhere to the plate. After centrifugation using a swing bucket rotor with a plate adaptor, you can remove the liquid by using a pipette.
- Is special software required for data analysis?
-
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). FCS files can be analyzed by different software. Data generated from using LEGENDplex™ kits can be analyzed using the LEGENDplex™ data analysis software freely available on the BioLegend website (biolegend.com/legendplex/software). A free of charge software license dongle is currently provided in the kit. Please check our website for the most updated versions of the software.
- Should I perform the assay with the filter plates or with V-bottom plates?
-
Filter plates or V-bottom plates have been included in some kits for your convenience. Performing the assay with filter plates is generally recommended. A vacuum filtration unit is required to work with the filter plates. If however, you don’t have access to a vacuum manifold, then you can use the V-bottom plates or micro-FACS tubes as described in the manual and follow the recommended assay protocols for the type of plates or tubes you choose. All tubes and plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- What is the shelf life of LEGENDplex™ kits?
-
LEGENDplex™ kits are valid for 6 months from the date of receipt.
