FITC anti-human/mouse Granzyme B Recombinant Antibody

Pricing & Availability
Clone
QA16A02 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA16A02_FITC_GranzymeB_Antibody_1_042817
Human peripheral blood mononuclear cells were stained with CD8 Pacific Blue™, fixed and permeabilized, and then stained with Granzyme B FITC (clone QA16A02, top) or mouse IgG1, κ FITC isotype control (bottom).
  • QA16A02_FITC_GranzymeB_Antibody_1_042817
    Human peripheral blood mononuclear cells were stained with CD8 Pacific Blue™, fixed and permeabilized, and then stained with Granzyme B FITC (clone QA16A02, top) or mouse IgG1, κ FITC isotype control (bottom).
  • QA16A02_FITC_GranzymeB_Antibody_2_042817
See FITC spectral data
Cat # Size Price Save
372205 25 tests ¥17,000
372206 100 tests ¥39,000
Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Product Citations
  1. Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
  2. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  3. Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed
  4. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  5. Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed
  6. Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed
  7. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  8. Sun Y, et al. 2020. Cell. 184(2):404-421.e16. PubMed
  9. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  10. Dong L, et al. 2021. Cancer Cell. . PubMed
  11. Su Y, et al. 2020. Cell. 1479:183. PubMed
  12. Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed
  13. Li M, et al. 2020. Virol Sin. 35:588. PubMed
RRID
AB_2687029 (BioLegend Cat. No. 372205)
AB_2687030 (BioLegend Cat. No. 372206)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Caspase-3
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
T cells, NK cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

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Go To Top Version: 0    Revision Date: 05/01/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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