C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
C57BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% rat serum, and stained with CD11c- BV421™(red), B220-Alexa Fluor® 647 (blue), and I-A/I-E-FITC (green) in 1% BSA and 0.1% Tween-20 in PBS. Images were acquired with an automated widefield microscope (Nikon Eclipse Ti) and a CCD camera (QImaging Retiga 2000R). Emitted light was collected through 440/40, 525/50, and 700/75 nm bandpass filters. Images provided by Ann Haberman and Christine Podolski, Yale University.
CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
Application References
(PubMed link indicates BioLegend citation)
Granucci F, et al. 1997. J. Immunol. 159:1794.
Stokes RW, et al. 1998. J. Immunol. 160:5514.
Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
Ma XT, et al. 2006. Cancer Research 66:1169.
Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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