- 6H6 (See other available formats)
- Regulatory Status
- Other Names
- IL-3Rα, IL-3 Receptor alpha
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CDw131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- Human IL-3Rα transfected COS cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.
(PubMed link indicates BioLegend citation)
- Sun Q, et al. 1996. Blood 87:83. (IP, WB)
- Herling M, et al. 2003. Blood 101:5007. (IHC)
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
- Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
AB_2904334 (BioLegend Cat. No. 306053)
AB_2904334 (BioLegend Cat. No. 306054)
- Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes
- Hematopoietic cell proliferation, differentiation
- Cell Type
- Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Miyajima A, et al. 1993. Blood 82:1960.
- Gene ID
- 3563 View all products for this Gene ID
- View information about CD123 on UniProt.org
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-human CD123
PE anti-human CD123
Purified anti-human CD123
PE/Cyanine5 anti-human CD123
PE/Cyanine7 anti-human CD123
APC anti-human CD123
FITC anti-human CD123
PerCP/Cyanine5.5 anti-human CD123
Brilliant Violet 421™ anti-human CD123
Brilliant Violet 650™ anti-human CD123
Brilliant Violet 510™ anti-human CD123
Alexa Fluor® 647 anti-human CD123
Brilliant Violet 605™ anti-human CD123
Purified anti-human CD123 (Maxpar® Ready)
Brilliant Violet 711™ anti-human CD123
Brilliant Violet 785™ anti-human CD123
PE/Dazzle™ 594 anti-human CD123
Alexa Fluor® 488 anti-human CD123
PE/Cyanine7 anti-human CD123
TotalSeq™-A0064 anti-human CD123
Alexa Fluor® 700 anti-human CD123
APC/Fire™ 750 anti-human CD123
Pacific Blue™ anti-human CD123
TotalSeq™-C0064 anti-human CD123
TotalSeq™-B0064 anti-human CD123
TotalSeq™-D0064 anti-human CD123
PerCP anti-human CD123
GMP PE/Cyanine7 anti-human CD123
APC/Fire™ 810 anti-human CD123 Antibody