APC anti-human CD254 (TRANCE, RANKL) Antibody

Pricing & Availability
MIH24 (See other available formats)
Regulatory Status
Other Names
TRANCE (TNF-related activation-induced cytokine receptor), CD254, RANK-L (receptor activator of NF-κB ligand), OPGL (Osteoprotegerin ligand), SOFA, TNFSF-11
Mouse IgG2b, κ
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Product Citations
Human RANKL transfected P815 cell line was stained with CD254 (clone MIH24) APC (filled histogram) or mouse IgG2b, κ APC isotype control (open histogram).
  • MIH24_APC_CD254_Antibody_FC_092514
    Human RANKL transfected P815 cell line was stained with CD254 (clone MIH24) APC (filled histogram) or mouse IgG2b, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Save
347507 25 tests ¥32,340
347508 100 tests ¥70,560

Human CD254, also known as RANKL, TRANCE, and OPGL, is a type II transmembrane protein in the TNF superfamily that has two forms: a membrane-anchored protein and a secreted protein. Its extracellular domain is related to TRAIL, FasL, and TNF. CD254 is expressed on osteoblasts and activated T cells. It has been implicated in the regulation of the interactions between T cells and dendritic cells. It plays an important role in osteoblast differentiation and bone resorption. CD254 activates JNK and NF-κB and induces the expression of IL-1, IL-6, IL-12, and IL-15 in dendritic cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
RANKL-transfected L cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: ELISA and immunohistochemical staining of paraffin-embedded tissue sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanamaru F, et al. 2004. Immunol. Lett. 94:239.
  2. Mueller A, et al. 2014. Arthritis Res. Ther. 16:R55. (IHC) PubMed
Product Citations
  1. Marini C,et al. 2017. Sci Rep. . 10.1038/s41598-017-12761-1. PubMed
AB_2564322 (BioLegend Cat. No. 347507)
AB_2564323 (BioLegend Cat. No. 347508)

Antigen Details

A TNF superfamily member.

Expressed on osteoblasts and activated T cells.

Plays an important role in osteoblast differentiation and bone resorption, regulates the interactions between T cells and dendritic cells, and T cells and B cells.
Cell Type
T cells
Biology Area
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Wiethe C, et al. 2003. J. Immunol. 171:4121
2. Page G, et al. 2005. Arthritis Rheum. 52:2307
3. Ji JD, et al. 2009. J. Immunol. 183:7223

Gene ID
8600 View all products for this Gene ID
View information about CD254 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/30/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • TotalSeq™-C0356 anti-human CD254 (TRANCE, RANKL)


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