Alexa Fluor 488 anti-Oct4 Oct3 Antibody anti-Oct4

Clone: 3A2A20 (See other available formats)
Regulatory Status: RUO
Other Names: POU domain class 5, transcription factor 1 (POU5F1), OCT4, OCT3
Isotype: Mouse IgG2b, κ
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3A2A20_AF488_OCT4_Antibody_FC_062413 — Human teratocarcinoma cell line NCCIT cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set, and then stained with anti-human OCT4 (clone 3A2A20) Alexa Fluor® 488 (filled histogram) or mouse IgG2b, κ Alexa Fluor® 488 isotype control (open histogram).

Compare all formats See Alexa Fluor® 488 spectral data

Cat #	Size	Price	Save
653705	25 tests	¥37,620
653706	100 tests	¥84,040

Description

Oct4 (Octamer binding transcription factor 4) is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells. When embryonic stem cells are triggered to differentiate, Oct4 is downregulated, thus providing a model for the early events linked to somatic differentiation in the developing embryo. Oct4 is a nuclear protein, with a predicted molecular weight of 39 kD.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human Oct4 recombinant protein (1-141aa).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
RRID: AB_2562250 (BioLegend Cat. No. 653705)
AB_2562251 (BioLegend Cat. No. 653706)

Antigen Details

Structure: Belongs to the POU transcription factor family, predicted molecular weight of 39 kD
Distribution: Nuclear protein, expressed in undifferentiated embryonal carcinoma (EC) cells, embryonic stem (ES) cells and embryonic germ (EG) cells
Function: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Oct4 is a prime candidate for an early developmental control gene. It also is an important marker of the undifferentiated state and central regulator of pluripotency in ES cells.
Interaction: SOX2, SOX8, SOX10, androgen receptor, and ETS2
Biology Area: Cell Biology, Immunology, Stem Cells, Transcription Factors
Antigen References: 1. Niwa H, et al. 2000. Nature Genet. 24:372.
2. Wernig M, et al. 2007. Nature 448:318.
3. Pesce M, et al. 2001. Stem Cells 19:271.
Gene ID: 5460 View all products for this Gene ID
UniProt: View information about Oct4 on UniProt.org

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Other Formats

View All Oct4 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Oct4 (Oct3)	3A2A20	WB,ICC,ChIP
PE anti-Oct4 (Oct3)	3A2A20	ICFC
Alexa Fluor® 488 anti-Oct4 (Oct3)	3A2A20	ICFC
Brilliant Violet 421™ anti-Oct4 (Oct3)	3A2A20	ICC,ICFC
Alexa Fluor® 647 anti-Oct4 (Oct3)	3A2A20	ICC,ICFC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

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