- W17199A (See other available formats)
- Regulatory Status
- Other Names
- Ciliary neurotrophic factor
- Rat IgG2a, λ
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- Product Citations
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CNTF, ciliary neurotrophic factor, was initially identified as a survival factor for cultured embryonic chick parasympathetic neurons. The human protein has 84% similarity to the rat and rabbit proteins. It lacks the N-terminal signal sequence and consensus sequences for glycosylation or hydrophobic regions. The CNTF profile shows similar sequence and structure motifs to hematopoietic cytokines (IL-6, IL-11, LIF, oncostatin M, cardiotrophin-1) and thus was considered a member of the IL-6 family. CNTF exerts effects on glial cells and neurons. CNTF activates astrocytes and promotes their capacity to support neurons and oligodendroglia. CNTF stimulates astrocytes to secrete FGF-2 and rat microglia to secrete glial cell line-derived neurotrophic factor (GDNF). CNTF is produced by astrocytes after brain injury. The CNTF receptor complex includes the CNTF binding protein (CNTFRα), the LIF binding protein (LIFRb), and the signal transducer of IL-6 (gp130). CNTFRα does not have transmembrane or intracellular domains, which means it does not induce signal transduction per se. CNTFRα is linked to the cell membrane via glycosyl phosphatidylinositol linkage, and it can be released by phospholipase C to produce a soluble receptor. The complex formed by CNTFRα and CNTF serve as agonists for cells that do not express CNTFRα. Cardiotrophin-like cytokine (CLC) also binds to CNTFRα, which proposed to the activation of gp130, which are LIFRb subunits. CNTF reduces the symptoms of experimental autoimmune encephalomyelitis, and its absence exacerbates the severity of multiple sclerosis disability. CNTF has been studied in retinal degenerative disorders and was shown as a potential clinical application.Product Details
- Antibody Type
- Host Species
- Recombinant mouse CNTF
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Neut - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by the inhibition of proliferation induced by recombinant mouse CNTF (200 ng/mL) on TF-1 cells. ND50 range: 0.15 -1 μg /mL. It is recommended that the reagent be titrated for optimal performance for each application.
Astrocytes, microglia, hepatocytes, skeletal muscle, embryonic stem cells, and bone marrow stromal cells
- Stimulates gene expression, along with cell survival or differentiation in neuronal cell types. It has a potent trophic factor for motor neurons. IL-1b, TNFα (TNF-α), and EGF induces the release of CNTF from astrocytes.
- Sensory, sympathetic, ciliary, motor neurons, microglia, retinal ganglion cells, adipocytes, and skeletal muscle cells
- Trimeric complex form by CNTF receptor-α (CNTFRα), LIF receptor (LIFR), and gp130.
- Antigen References
1. Negro A, et al. 1991. Eur. J. Biochem. 201:289.
2. Bazan JF. 1991. Neuron. 7:197.
3. Davis S, et al. 1993. Science. 260:1805.
4. Plun-Favreau H, et al. 2001. EMBO J. 20:1692.
5. Lin HW, et al. 2009. J. Neuroinflammation 6:7.
6. Wen R, et al. 2012. Prog. Retin. Eye. Res. 31:136.
- Gene ID
- 12803 View all products for this Gene ID
- View information about CNTF on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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