Ultra-LEAF™ Purified anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
W18349F (See other available formats)
Regulatory Status
RUO
Other Names
High affinity immunoglobulin gamma Fc receptor I, IgG Fc receptor I, Fc gamma RI, FcRI, CD64, Fcg1, FcgR1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
Biotin mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400204) (black circles) binds to immobilized recombinant mouse FcγRI/CD64 (Cat. No. 773806). Ultra-LEAF™ purified anti-mouse CD64 (FcγRI) antibody (clone W18349F) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400666) (gray triangles) does not have the effect. This antibody blocks the binding of 2 µg/mL biotin mouse IgG2a, κ to 1 µg/mL immobilized recombinant mouse FcγRI/CD64. ND50 = 0.02 - 0.1 µg/mL.
  • Biotin mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400204) (black circles) binds to immobilized recombinant mouse FcγRI/CD64 (Cat. No. 773806). Ultra-LEAF™ purified anti-mouse CD64 (FcγRI) antibody (clone W18349F) (purple squares) inhibits the binding in a dose-dependent manner whereas the GoInVivo™ purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400666) (gray triangles) does not have the effect. This antibody blocks the binding of 2 µg/mL biotin mouse IgG2a, κ to 1 µg/mL immobilized recombinant mouse FcγRI/CD64. ND50 = 0.02 - 0.1 µg/mL.
Cat # Size Price Quantity Avail. Save
941903 100 µg 312,00€
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941904 1 mg 1520,00€
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Description

FcγRI, also known as CD64 and FcR I, is a type I transmembrane protein, belonging to the immunoglobulin superfamily. FcγRI is an Fc receptor that binds to monomeric IgG with high affinity. Human FcγRI binds to human IgG1, IgG3 and IgG4 with higher affinity, but no affinity for IgG2. However, murine FcγRI only binds to mouse IgG2a with high affinity, but it binds to mouse IgG2b and IgG3 with low affinity and no affinity for mouse IgG1. Upon binding to IgG, FcγRI associates with signal-transducing FcR common γ chain, and in turn conducts downstream signaling. Though Fc γ chain is dispensable to FcγRI ligand-binding capacity, the surface expression of FcγRI and signaling are impaired in Fc γ chain-deficient cells. FcγRI is exclusively expressed on myeloid cells, including monocytes/macrophages, dendritic cells, and inducible on neutrophils and mast cells. The expression can be upregulated by IFN-γ, IL-10 and G-CSF stimulation. Several studies demonstrated that FcγRI plays a role in antigen capture, IgG-induced cellular phagocytosis and antibody-dependent cellular cytotoxicity (ADCC). In FcγRI-deficient mouse macrophages, the IgG2a-induced phagocytosis and antibody-mediated killing were impaired. In addition, acute and chronic inflammatory responses and immune complex-dependent antigen presentation to primed T cells were disturbed in FcγRI-deficient mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD64 (FcγRI)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 2 µg/mL biotin mouse IgG2a to 1 µg/mL immobilized recombinant mouse FcγRI/CD64 (Cat. No. 773806). ND50 range: 0.02 – 0.1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Immunoglobulin family
Distribution

Monocytes, macrophages, dendritic cells and activated granulocytes

Function
Antigen capture, IgG-induced cellular phagocytosis, antibody-dependent cellular cytotoxicity
Interaction
FcγR subunit, src-type kinase Hck and Lyn, dendritic cells, granulocytes, macrophages, monocytes
Ligand/Receptor
Mouse IgG2a
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Van de Winkel JG, et al. 1993. Immunol Today. 14:215-21.
  2. Raghaven M, et al. 1996. Annu Rev Cell Dev Biol. 12:181-220.
  3. Ravetch J, et al. 2001. Annu Rev Immunol. 19:275-90.
  4. Takai T. 2002. Nature Rev Immunol. 2:580-92.
  5. Ravetch J, et al. 2000. Science. 290:84-9.
Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/25/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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