- EH12.2H7 (See other available formats)
- Regulatory Status
- Other Names
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
This product is eligible for a 40% discount! Promotion valid until December 31st, 2022. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey
- Antibody Type
- Host Species
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
Block, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
- Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
- Velu V, et al. 2007. J. Virol. 81:5819. (FA)
- Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
- Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
- Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
- Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
- Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
- Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
- Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
- Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
- Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_11147369 (BioLegend Cat. No. 329925)
AB_11147365 (BioLegend Cat. No. 329926)
AB_2783202 (BioLegend Cat. No. 329957)
AB_2783203 (BioLegend Cat. No. 329958)
AB_2783204 (BioLegend Cat. No. 329959)
AB_2783205 (BioLegend Cat. No. 329960)
- Immunoglobulin superfamily
Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells
- B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
- Cell Type
- B cells, Lymphocytes, T cells, Thymocytes, Tregs
- Biology Area
- Cancer Biomarkers, Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Gene ID
- 5133 View all products for this Gene ID
- View information about CD279 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Brilliant Violet 421™ anti-human CD279 (PD-1)
Purified anti-human CD279 (PD-1)
FITC anti-human CD279 (PD-1)
PE anti-human CD279 (PD-1)
APC anti-human CD279 (PD-1)
Alexa Fluor® 647 anti-human CD279 (PD-1)
PerCP/Cyanine5.5 anti-human CD279 (PD-1)
APC/Cyanine7 anti-human CD279 (PD-1)
Pacific Blue™ anti-human CD279 (PD-1)
PE/Cyanine7 anti-human CD279 (PD-1)
Purified anti-human CD279 (PD-1) (Maxpar® Ready)
Brilliant Violet 605™ anti-human CD279 (PD-1)
Ultra-LEAF™ Purified anti-human CD279 (PD-1)
Brilliant Violet 711™ anti-human CD279 (PD-1)
Brilliant Violet 785™ anti-human CD279 (PD-1)
Brilliant Violet 510™ anti-human CD279 (PD-1)
Biotin anti-human CD279 (PD-1)
PE/Dazzle™ 594 anti-human CD279 (PD-1)
Alexa Fluor® 488 anti-human CD279 (PD-1)
PerCP anti-human CD279 (PD-1)
GoInVivo™ Purified anti-human CD279 (PD-1)
Brilliant Violet 650™ anti-human CD279 (PD-1)
Alexa Fluor® 700 anti-human CD279 (PD-1)
APC/Fire™ 750 anti-human CD279 (PD-1)
TotalSeq™-A0088 anti-human CD279 (PD-1)
TotalSeq™-B0088 anti-human CD279 (PD-1)
TotalSeq™-C0088 anti-human CD279 (PD-1)
Brilliant Violet 750™ anti-human CD279 (PD-1)
TotalSeq™-D0088 anti-human CD279 (PD-1)
PE/Fire™ 640 anti-human CD279 (PD-1)
PE/Cyanine5 anti-human CD279 (PD-1)