Ultra-LEAF™ Purified anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
W18063F (See other available formats)
Regulatory Status
RUO
Other Names
CD137, CDw137, 4-1BB, 4-1BB ligand receptor, T cell antigen ILA, T cell antigen 4-1BB homolog
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W18063F_ULEAF_CD137_4-1BB_Antibody_071621.png
Recombinant human TNFRSF9/4-1BB-Fc Chimera (Cat. No. 772606) (black circles) binds to immobilized recombinant human TNFSF9 (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137 (4-1BB) antibody (clone W18063F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.03 µg/mL recombinant human TNFRSF9/4-1BB-Fc Chimera to 0.5 µg/mL of immobilized recombinant human TNFSF9 with an ND50 = 0.01 - 0.06 µg/mL.
  • W18063F_ULEAF_CD137_4-1BB_Antibody_071621.png
    Recombinant human TNFRSF9/4-1BB-Fc Chimera (Cat. No. 772606) (black circles) binds to immobilized recombinant human TNFSF9 (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137 (4-1BB) antibody (clone W18063F) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 0.03 µg/mL recombinant human TNFRSF9/4-1BB-Fc Chimera to 0.5 µg/mL of immobilized recombinant human TNFSF9 with an ND50 = 0.01 - 0.06 µg/mL.
Cat # Size Price Quantity Avail. Save
944903 100 µg 520,00€
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944904 1 mg 1752,00€
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Description

TNFRSF9, also known as 4-1BB and CD137, is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It is a costimulatory receptor similar to OX40, CD27, CD30, and CD40. The interaction of TNFRSF9 and its ligand, TNFSF9, leads to the activation of cytotoxic T cells, reactivation of anergic T lymphocytes, and induces T cell proliferation. TNFRSF9/TNFSF9 preferentially enhances the expansion of CD8+ T cells rather than CD4+ T cells, through the increase of IL-2Rα (CD25) and IL-2 expressions in CD8+ T cells. Among the TNFRSF members, TNFRSF9 is the most potent inducer of IL-2Rα expression on CD8+ T cells. In vivo studies with TNFSF9 knockout mice and with the agonistic anti-TNFRSF9 monoclonal antibody showed that TNFRSF9/TNFSF9 interaction keeps the optimal CD8+ T cell-mediated immune response against viral infections and tumors. The mouse TNFRSF9 has 60% identity to the human receptor at the amino acid level.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD137
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between human TNFSF9 (Cat. No. 750006) and human TNFRSF9/4-1BB (Cat. No. 772606). The ND50 range: 0.01 - 0.06 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations

Antigen Details

Structure
Dimer
Distribution

Activated T cells, natural killer cells, macrophages, neutrophils, dendritic cells, eosinophils, Tregs, activated B cells, mast cells, and endothelial cells in tumor capillaries

Function
Proliferation of T cells, activates their effector functions, survival, and establishes immunological memory
Interaction
Activated antigen presenting cells, macrophages, B cells, B cell lymphoma, dendritic cells, a small fraction of T cells, epithelial, and endothelial cells.
Ligand/Receptor
TNFSF9 and CD137L
Cell Type
B cells, Dendritic cells, Endothelial cells, Mast cells, T cells, Tregs
Biology Area
Immuno-Oncology, Immunology, Protein Trafficking and Clearance
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Pollok KE, et al. 1994. Eur. J. Immunol. 24:367.
  2. Watts TH. 2005. Annu. Rev. Immunol. 23:23-68.
  3. Zhu Y, et al. 2007. Blood 109:4882.
  4. Locatelli F2009. Blood 11:2208.
  5. Wang J, et al. 2010. J. Immunol. 185:7654.
  6. Eun SY, et al. 2015. J. Immunol. 1:134-41.
  7. Spencer AJ, et al. 2014. PLoS One. 9:e105520.
  8. Yi L, et al. 2014. PLoS One 1:e86337.
Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/16/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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