Recombinant Mouse G-CSF (mammalian expressed, carrier-free)

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Other Names
CSF3, CSFG, MGI-IG, Granulocyte colony stimulating factor
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G-CSF_Mouse_RECOM_Mammilianexpressed_CF_BA_070115
Mouse G-CSF induces the proliferation of mouse myelogenous leukemia lymphoblast M-NFS-60 cell line.
  • G-CSF_Mouse_RECOM_Mammilianexpressed_CF_BA_070115
    Mouse G-CSF induces the proliferation of mouse myelogenous leukemia lymphoblast M-NFS-60 cell line.
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Cat # Size Price Quantity Avail. Save
752102 10 µg 296€
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752104 25 µg 472€
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752106 100 µg 1.224€
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752108 500 µg 3.192€
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Description

G-CSF is the key hematopoietic cytokine involved in the control of neutrophil production and therefore is a critical regulator of the innate immunity against bacterial infections. G-CSF mobilizes stem cells indirectly by down-regulating the expression of CXCL12 on marrow osteoblasts and by releasing neutrophils and monocyte proteolytic enzymes, which include neutrophil elastase, cathepsin G, and MMP9. In turn, they degrade important HSC-trafficking and adhesion molecules including c-kit, CXCR4, VCAM-1(CD106), fibronectin, and OPN, which leads to reduced cellular adhesion of HSC through their receptor VLA-4 to bone marrow stroma. In addition, G-CSF possesses immunosuppressive effects on monocytes/ macrophages, dendritic cells, and T lymphocytes. The receptor for G-CSF is expressed not only in hematopoietic cells, but is also expressed on cardiomyocytes, skeletal muscle, and neurons. G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. In vitro, G-CSF displays strong antiapoptotic activity in neuronal cells. The beneficial effects of G-CSF have been demonstrated in rodent stroke models. G-CSF is used in patients with leukocytopenia and in donors of peripheral blood-derived hematopoietic progenitor cells prior to collection for transplantation.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse G-CSF, amino acids (Val31-Ala208) (Accession# NM_009971), was expressed in 293E cells.
Molecular Mass
The 178 amino acid recombinant protein has a predicted molecular mass of approximately 18.9 kD. The DTT-reduced and non-reduced protein migrates at approximately at 23-26 kD by SDS-PAGE. The N-terminal amino acid is Val.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.05 - 0.25 ng/ml, corresponding to a specific activity of 4 - 20 x 106 units/mg, as determined by M-NFS-60 cell proliferation induced by mouse G-CSF in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homotrimer.
Distribution
Monocytes, mesothelial cells, fibroblasts, endothelial cells, and neurons.
Function
G-CSF acts on myeloid progenitor cells to stimulate survival, proliferation, and neutrophilic maturation. On mature neutrophils, it enhances survival, superoxide anion and alkaline phosphatase production, arachidonic acid release, and antibody dependent cellular cytotoxicity.
Interaction
Hematopoietic progenitor cells, neutrophilic granulocytes, monocytes, macrophages, osteo macrophages, platelets, endothelial cells, trophoblastic cells, myoblasts, cardiomyocytes, neurons, and some small cell lung carcinoma cell lines.
Ligand/Receptor
G-CSFR (CD114)
Cell Type
Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Levesque J, et al. 2001. Blood 98:1289.
2. Kim HK, et al. 2006. Blood 108:812.
3. Donahue RE, et al. 2009. Blood 114:2530.
4. Shimoji K, et al. 2010. Cell Stem Cell 6:227.
5. Martins A, et al. 2010. IUBMB Life 8:611.
6. Hara M, et al. 2011. J. Exp. Med. 208:715.
7. Bonig H and Papayannopoulou T. 2012. Methods Mol. Biol. 904:1.
8. Kadota R, et al. 2012. PLoS One 7(11):e50391.

Gene ID
12985 View all products for this Gene ID
UniProt
View information about G-CSF on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 07/02/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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