Recombinant Mouse CXCL3 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Gro3, Groγ, Small inducible cytokine subfamily B, member 3 (Scyb3), macrophage inflammatory protein-2 beta (MIP2B), CINC-2b
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Product Citations
publications
CXCL3_Mouse_Recombinant_Protein_BA_091213
Human neutrophils chemoattracted by mouse CXCL3.
  • CXCL3_Mouse_Recombinant_Protein_BA_091213
    Human neutrophils chemoattracted by mouse CXCL3.
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590802 10 µg 200,00€
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Description

CXCL3 is an ELR CXC chemokine, and it is structurally and functionally related to GRO1 (CXCL1), GRO2 (CXCL2), and interleukin-8 (CXCL8). CXCL3 binds to the CXCR2 receptor, and this receptor is shared with other ELR CXC chemokines (CXCL1, CXCL2, CXCL5, CXCL6, CXCL7, and CXCL8). CXC chemokines play a crucial role in the first phase of inflammation; in this stage, PMN cells are rapidly chemoattracted. In the next step of inflammation, the CC chemokines (MCPs) attract different cell subpopulations such as T cells, monocytes, basophils, and eosinophils. MMP12, mainly produced by macrophages, modulates the activity of ELR-CXC chemokines, and it cleaves human CXCL1, CXCL2 and CXCL3 within the ELR sequence at Glu6-Leu7. The ELR sequence is critical in receptor binding; therefore, the cleavage inactivates these chemokines and abrogates the PMN influx. CXCL3 is expressed in colon carcinoma, and it is associated with metastasis.  Also, CXCL1, CXCL2 and CXCL3 have been shown to be highly expressed in patients with malignant melanoma. CXCL3, in addition to other chemokines, is induced by IL-17A in psoriasis. Also, CXCL3 and other pro-angiogenic ELR+ chemokines are induced by microparticules in synovial fibroblasts from patients with rheumatoid arthritis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse CXCL3, amino acids Ala28-Ser100 (Accession# NM_203320.3) was expressed in E. coli.
Molecular Mass
The 73 amino acid recombinant protein has a predicted molecular mass of approximately 7.9 kD. The DTT-reduced and non-reduced protein migrate at approximately 10 and 12 kD by SDS-PAGE respectively. The N-terminal amino acid is Ala.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 100 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Product Citations
  1. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed

Antigen Details

Structure
Chemokine.
Distribution

Macrophages, monocytes, fibroblasts, epithelial cells, keratinocytes, and placental trophoblats.

Function
CXCL3 chemoattracts and activates neutrophils. CXCL3 is induced by IL-1a, IL-17A, and GnRH. CXCL3 is inactivated by MMP12.
Interaction
Neutrophils, basophils, lymphocytes, monocytes, keratinocytes, endothelial cells, epithelial cells, fibroblasts, melanoma cells, and melanocytes.
Ligand/Receptor
CXCR2.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Signal Transduction, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Haskill S, et al. 1990. Proc. Natl. Acad. Sci. USA 87:7732.
2. Dean RA, et al. 2008. Blood 112:3455.
3. Cavanagh PC, et al. 2009. Am. J. Physiol. Cell Physiol. 297:C17.
4. Doll D, et al. 2010. Int. J. Colorectal. Dis. 25:573.
5. Keeley EC, et al. 2011. Exp. Cell Res. 317:685.
6. Reich N, et al. 2011. J. Cell Mol. Med. 15:756.
7. Girolomoni G, et al. 2012. Br. J. Dermatol. 167:717.

Gene ID
330122 View all products for this Gene ID
UniProt
View information about CXCL3 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 2    Revision Date: 09/12/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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