Recombinant Mouse CCL9 (MIP-1γ) (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
CCF18, Macrophage inflammatory protein (MIP)-related protein-2 (MRP-2)
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CCL9_Mouse_Recombinant_Protein_BA_012413
Mouse CCL9 chemoattract Baf3-hCCR1 transfectants .
  • CCL9_Mouse_Recombinant_Protein_BA_012413
    Mouse CCL9 chemoattract Baf3-hCCR1 transfectants .
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586202 10 µg 200,00€
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Description

Mouse CCL9 was initially cloned from cDNA libraries of BaF3 and RAW 264.7 cells. It is constitutively expressed in a big variety of tissues, including the follicle-associated epithelium overlaying the dome regions of the Peyer’s patches. Using CCR6 -/- mice, it was shown that CCL9 recruits CD11b+ dendritic cells (DCs) to the sub-epithelial dome regions of the Peyer’s patch. This data showed that the CCL20/CCR6 pair is not absolutely required for the recruitment of CD11b+ DCs to that region. In addition, CCL9 is induced in Raw264.7 and bone marrow cells by RANKL, and the use of this osteoclastogenesis model suggested that CCL9 stimulates the activation and survival of mature osteoclasts. The expression of CCL9 and CCR1 in osteoclasts suggests a role for CCL9 in bone resorption. Additional data have shown that activation of TLR-9 in microglia with unmethylated CpG oligodeoxynucleotides (CpG-ODNs) induces production of CCL9 and expression of CCR1. In addition, Retinoid X Receptor alpha (RXRa) plays a role in the regulation of the innate immune response, and the disruption of this nuclear receptor, in peritoneal macrophages, induces a decrease in CCL6 and CCL9 in vivo, correlating with impaired leukocyte recruitment to inflammatory sites and prolonged survival in sepsis.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse CCL9, amino acids Gln22-Gln122 (Accession# NM_011338.2) was expressed in E. coli.
Molecular Mass
The 101 amino acid recombinant protein has a predicted molecular mass of approximately 11.5 kD. The DTT-reduced protein migrates at approximately 12 kD and non-reduced protein migrates at approximately 14.5 kD by SDS-PAGE. The N-terminal amino acid is Gln.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract Baf3-hCCR1 transfectants in a dose dependent manner. The ED50 = 2 - 10 ng/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Chemokine
Distribution

Macrophages, microglia, monocytes, osteoclasts, Langerhans cells, DCs, Sertoli cells, and follicle associated epithelium

Function
CCL9 chemoattracts T cells, monocytes, and dendritic cells. It suppresses colony formation by murine and human bone marrow myeloid progenitor cells and regulates bone reabsorption. CCL9 is induced by RANKL during osteoclastogenesis. CCL9 is also induced by TLR-9 activation.
Interaction
CD4 and CD8 T cells, monocytes, osteoclasts, CD11b+ dendritic cells
Ligand/Receptor
CCR1
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Hara T, et al. 1995. J. Immunol. 155:5352.
2. Zhao X, et al. 2003. J. Immunol. 171:2797.
3. Okamatsu Y, et al. 2004. J. Immunol. 173:2084.
4. Ravindran C, et al. 2010. Cell Immunol. 260:113.
5. Nuñez V, et al. 2010. P. Natl. Acad. Sci. USA 107:10626.
6. Simon L, et al. 2010. Stem Cells 28:1882.

Gene ID
20308 View all products for this Gene ID
UniProt
View information about CCL9 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 1    Revision Date: 01/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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