Purified anti-mouse IL-17A (Maxpar® Ready) Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
TC11-18H10.1_Purified_IL-17A_CyTOF_1_112213
Mouse EL4 T cells were incubated for 5 hours in media alone (bottom) or with PMA and Ionomycin (top) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 147Sm-anti-CD45 (30-F11) and 169Tm-anti-IL17A (TC11-18H10.1). Data provided by DVS Sciences.
  • TC11-18H10.1_Purified_IL-17A_CyTOF_1_112213
    Mouse EL4 T cells were incubated for 5 hours in media alone (bottom) or with PMA and Ionomycin (top) in the presence of monensin and brefeldin A. Cells were then fixed, permeabilized, and stained with 147Sm-anti-CD45 (30-F11) and 169Tm-anti-IL17A (TC11-18H10.1). Data provided by DVS Sciences.
  • TC11-18H10.1_Purified_IL-17A_CyTOF_2_112213
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506935 100 µg 256€
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Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. McDonald B, et al. 2020. Cell Host Microbe. 28(5):660-668.e4. PubMed
RRID
AB_2562850 (BioLegend Cat. No. 506935)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.
Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All IL-17A Reagents Request Custom Conjugation
Description Clone Applications
PE/Dazzle™ 594 anti-mouse IL-17A TC11-18H10.1 ICFC
APC/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 711™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP anti-mouse IL-17A TC11-18H10.1 ICFC
PE anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture, CyTOF®, ELISPOT Capture, ICFC
Ultra-LEAF™ Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture, CyTOF®, ELISPOT Capture, Neut, ICFC
LEAF™ Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture, CyTOF®, ELISPOT Capture, Neut, ICFC
FITC anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 488 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 647 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 700 anti-mouse IL-17A TC11-18H10.1 ICFC
APC anti-mouse IL-17A TC11-18H10.1 ICFC
Pacific Blue™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP/Cyanine5.5 anti-mouse IL-17A TC11-18H10.1 ICFC
PE/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 421™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 605™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 650™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 785™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 510™ anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A (Maxpar® Ready) TC11-18H10.1 ELISA Capture, CyTOF®
Go To Top Version: 4    Revision Date: 07/22/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE/Dazzle™ 594 anti-mouse IL-17A

  • APC/Cyanine7 anti-mouse IL-17A

  • Brilliant Violet 711™ anti-mouse IL-17A

  • PerCP anti-mouse IL-17A

  • PE anti-mouse IL-17A

  • Purified anti-mouse IL-17A

  • Ultra-LEAF™ Purified anti-mouse IL-17A

  • LEAF™ Purified anti-mouse IL-17A

  • FITC anti-mouse IL-17A

  • Alexa Fluor® 488 anti-mouse IL-17A

  • Alexa Fluor® 647 anti-mouse IL-17A

  • Alexa Fluor® 700 anti-mouse IL-17A

  • APC anti-mouse IL-17A

  • Pacific Blue™ anti-mouse IL-17A

  • PerCP/Cyanine5.5 anti-mouse IL-17A

  • PE/Cyanine7 anti-mouse IL-17A

  • Brilliant Violet 421™ anti-mouse IL-17A

  • Brilliant Violet 605™ anti-mouse IL-17A

  • Brilliant Violet 650™ anti-mouse IL-17A

  • Brilliant Violet 785™ anti-mouse IL-17A

  • Brilliant Violet 510™ anti-mouse IL-17A

  • Purified anti-mouse IL-17A (Maxpar® Ready)

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