Purified anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
N418_A_Purified_Pos_071006.jpg
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC
  • N418_A_Purified_Pos_071006.jpg
    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC
  • N418_B_Purified_Neg_071006.jpg
Cat # Size Price Quantity Avail. Save
117301 50 µg 48,00€
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117302 500 µg 192,00€
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IP, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).

Application References

(PubMed link indicates BioLegend citation)
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
Product Citations
  1. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  2. Leppin K, et al. 2014. Invest Ophthalmol Vis Sci . 55:3603. PubMed
  3. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  4. Aparicio-Domingo P, et al. 2015. J Exp Med. 101084/jm20150318. PubMed
  5. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  6. Cepeda JR, et al. 2020. MAbs. 12:1836714. PubMed
  7. Martinez LE, et al. 2020. J Virol. 94:00:00. PubMed
  8. Tal MC, et al. 2020. MBio. 11:00. PubMed
  9. Mendes A, et al. 2021. Life Sci Alliance. 4:00. PubMed
  10. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  11. Molica F,et al. 2017. Sci Rep.. 10.1038/s41598-017-14130-4. PubMed
  12. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  13. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  14. Sutton C, et al. 2017. Nat Commun.. 10.1038/s41467-017-02111-0. PubMed
  15. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  16. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  17. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  18. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  19. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  20. Fu H, et al. 2019. Front Immunol. 10:271. PubMed
  21. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  22. Zhang Z, et al. 2018. Nat Med. 24:617. PubMed
  23. Hung LY, et al. 2019. J Immunol. 203:511. PubMed
  24. Simmons S et al. 2019. Elife. 8 pii: e41239. PubMed
  25. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  26. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  27. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  28. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  29. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  30. Kitai Y, et al. 2017. J Immunol. 198:1649. PubMed
  31. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  32. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  33. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  34. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  35. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  36. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  37. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  38. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  39. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  40. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  41. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  42. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  43. Sun H, et al. 2018. J Cell Biol. 217:1453. PubMed
  44. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  45. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  46. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  47. Ito Y, et al. 2018. J Exp Med. 215:2617. PubMed
  48. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  49. Bhatt K, et al. 2018. mSphere. 3:e00352. PubMed
  50. Bonnefoy F, et al. 2018. Front Immunol. 2.170833333. PubMed
  51. Dubrot J, et al. 2018. Life Sci Alliance. 1:e201800164. PubMed
  52. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  53. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  54. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  55. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  56. Borges TJ, et al. 2018. Nat Commun. 9:3482. PubMed
  57. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  58. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  59. Field CS, et al. 2018. Oncoimmunology. 7:e1376154. PubMed
  60. von Roemeling CA, et al. 2020. Nat Commun. 11:1508. PubMed
  61. Mazor R, et al. 2018. Proc Natl Acad Sci U S A. 115:E733. PubMed
  62. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  63. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  64. Hitchcock JR, et al. 2020. FEBS J. 287:250. PubMed
  65. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  66. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  67. Nascimento DC, et al. 2017. Nat Commun. 8:14919. PubMed
  68. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
  69. Trittel S, et al. 2019. Sci Rep. 9:16362. PubMed
  70. Mainini F, et al. 2018. Nucleic Acid Ther. 28:225. PubMed
  71. Wei S, et al. 2019. Virulence. 0.588888889. PubMed
  72. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  73. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  74. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  75. Sun L, et al. 2020. Nat Commun. 2.229166667. PubMed
  76. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  77. Majer O, et al. 2019. Nature. 575:366. PubMed
  78. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
  79. Cá B, et al. 2019. Front Microbiol. 1.876388889. PubMed
  80. Wikstrom M, et al. 2006. J Immunol. 177:913. PubMed
  81. Benson M, et al. 2007. J Exp Med. 204:1765. PubMed
  82. Pericolini E, et al. 2008. J Leukoc Biol. 83:1286. PubMed
  83. Osterholzer J, et al. 2009. J Immunol. 183:8044. PubMed
  84. Fahlén-Yrlid L, et al. 2009. J Immunol. 183:5032. PubMed
  85. Eisenach P, et al. 2010. J Cell Sci. 123:4182. PubMed
RRID
AB_313770 (BioLegend Cat. No. 117301)
AB_313771 (BioLegend Cat. No. 117302)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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