- APA1/1 (See other available formats)
- Regulatory Status
- Other Names
- CD3e molecule, epsilon (CD3-TCR complex), CD3e antigen, CD3 epsilon
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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The CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex. This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α/β or γ/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Purified denatured CD3 proteins from human thymus
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ICFC - Quality tested
WB, IHC-P - Verified
in vivo cell tracking - Reported in the literature, not verified in house
ICC, IHC, IP - Reported by the developer, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For Western blotting, the suggested use of this reagent is 1.0 - 4.0 µg per mL. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone APA1/1 recognizes an activation-dependent intracellular epitope of the CD3e chain. Additional reported applications (for the relevant formats of this clone) include: immunofluorescent staining of paraformaldehyde fixed sections1, in vivo tracking of T cells 2, and Western blotting3. The LEAF purified format is recommended for in vivo studies.
(PubMed link indicates BioLegend citation)
- Salmerón A, et al. 1991. J. Immunol. 147:3047. (ICFC, IF)
- Risueño RM, et al. 2005. Blood. 106:601. (IF, in vivo tracking, Mouse Reactivity)
- Risueño RM, et al. 2008. PLos One. 3:e1747. (WB)
- Gil D, et al. 2005. J. Exp. Med. 201:517.
- Risueño RM, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:9625.
- Product Citations
AB_2563713 (BioLegend Cat. No. 362701)
AB_2563714 (BioLegend Cat. No. 362702)
- Type I membrane protein, part of the TCR-CD3 complex. 207 amino acids, predicted molecular weight of 23 kD.
Mature T cells.
- Important role in T cell development and in linking antigen recognition with intracellular signaling pathways.
- Interacts with Nck.
- MHC antigen.
- Cell Type
- T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules, TCRs
- Antigen References
1. Savage PD, et al. 1988. Cytogenet. Cell. Genet. 49:289.
2. DeJarnette JB, et al. 1998. Proc. Natl. Acad. Sci USA. 95:14909.
3. Wang B, et al. 1999. J. Immunol. 162:88.
4. Szymczak AL, et al. 2005. J. Immunol. 175:270.
5. Borroto A, et al. 2014. J. Immunol. 192:2042.
- Gene ID
- 916 View all products for this Gene ID
- View information about CD3epsilon on UniProt.org
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