- HIR2 (See other available formats)
- Regulatory Status
- VII 70299
- Other Names
- Glycophorin A/B, GPA/GPB
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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The HIR2 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is the major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The HIR2 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.Product Details
- Verified Reactivity
- Human, African Green, Baboon
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
(PubMed link indicates BioLegend citation)
- Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
AB_2562825 (BioLegend Cat. No. 306615)
- Sialoglycoprotein, 20 kD
- Cell Type
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Mason D, et al. Eds. 2002. Leucocyte Typing VII. Oxford University Press. New York.
- Gene ID
- 2993 View all products for this Gene ID
- View information about CD235ab on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
|APC anti-human CD235ab||HIR2||FC|
|PE anti-human CD235ab||HIR2||FC|
|PE/Cyanine5 anti-human CD235ab||HIR2||FC|
|Purified anti-human CD235ab||HIR2||FC, CyTOF®, IHC-P|
|FITC anti-human CD235ab||HIR2||FC|
|Pacific Blue™ anti-human CD235ab||HIR2||FC|
|PerCP/Cyanine5.5 anti-human CD235ab||HIR2||FC|
|Purified anti-human CD235ab (Maxpar® Ready)||HIR2||FC, CyTOF®|
|Biotin anti-human CD235ab||HIR2||FC|
|PE/Cyanine7 anti-human CD235ab||HIR2||FC|
|APC/Fire™ 750 anti-human CD235ab||HIR2||FC|
|TotalSeq™-A0196 anti-human CD235ab||HIR2||PG|
|TotalSeq™-C0196 anti-human CD235ab||HIR2||PG|
|TotalSeq™-B0196 anti-human CD235ab||HIR2||PG|
|TotalSeq™-D0196 anti-human CD235ab||HIR2||PG|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD235ab
PE anti-human CD235ab
PE/Cyanine5 anti-human CD235ab
Purified anti-human CD235ab
FITC anti-human CD235ab
Pacific Blue™ anti-human CD235ab
PerCP/Cyanine5.5 anti-human CD235ab
Purified anti-human CD235ab (Maxpar® Ready)
Biotin anti-human CD235ab
PE/Cyanine7 anti-human CD235ab
APC/Fire™ 750 anti-human CD235ab
TotalSeq™-A0196 anti-human CD235ab
TotalSeq™-C0196 anti-human CD235ab
TotalSeq™-B0196 anti-human CD235ab
TotalSeq™-D0196 anti-human CD235ab