- 11F6 (See other available formats)
- Regulatory Status
- Other Names
- Mki67, Ki67, Ki-67, MIB-1, KIA
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.Product Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Host Species
- E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Excitation Laser
Blue Laser (488 nm)
AB_2910307 (BioLegend Cat. No. 151221)
AB_2910307 (BioLegend Cat. No. 151222)
- 325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Nucleus and chromosomes.
- Required for cell cycle progression and proliferation.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
- Starborg M, et al. 1996. J. Cell. Sci. 109:143.
- Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
- Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
- Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
- Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
- Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
- Gene ID
- 4288 View all products for this Gene ID 17345 View all products for this Gene ID
- View information about Ki-67 on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-mouse/human Ki-67||11F6||IHC-F, ICC|
|Alexa Fluor® 488 anti-mouse/human Ki-67||11F6||ICFC, ICC, IHC-F|
|Alexa Fluor® 647 anti-mouse/human Ki-67||11F6||ICFC, ICC, IHC-F|
|Brilliant Violet 421™ anti-mouse/human Ki-67||11F6||ICFC, IHC-F|
|PE anti-mouse/human Ki-67||11F6||ICFC|
|Alexa Fluor® 594 anti-mouse/human Ki-67||11F6||IHC-F|
|FITC anti-mouse/human Ki-67||11F6||ICFC|
|Brilliant Violet 650™ anti-mouse/human Ki-67||11F6||ICFC|
|PE/Cyanine7 anti-mouse/human Ki-67||11F6||ICFC|
|PE/Dazzle™ 594 anti-mouse/human Ki-67||11F6||ICFC|
|PerCP/Cyanine5.5 anti-mouse/human Ki-67||11F6||ICFC|
|Pacific Blue™ anti-mouse/human Ki-67||11F6||ICFC|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-mouse/human Ki-67
Alexa Fluor® 488 anti-mouse/human Ki-67
Alexa Fluor® 647 anti-mouse/human Ki-67
Brilliant Violet 421™ anti-mouse/human Ki-67
PE anti-mouse/human Ki-67
Alexa Fluor® 594 anti-mouse/human Ki-67
FITC anti-mouse/human Ki-67
Brilliant Violet 650™ anti-mouse/human Ki-67
PE/Cyanine7 anti-mouse/human Ki-67
PE/Dazzle™ 594 anti-mouse/human Ki-67
PerCP/Cyanine5.5 anti-mouse/human Ki-67
Pacific Blue™ anti-mouse/human Ki-67