PE/Fire™ 810 anti-mouse CD103 Recombinant Antibody

Pricing & Availability
Clone
QA17A24 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA17A24_PE-Fire-810_CD103_Antibody_060722
C57BL/6 splenocytes were stained with anti-mouse CD3 Alexa Fluor® 488 and anti-mouse CD103 recombinant (clone QA17A24) PE/Fire™ 810 or stained with anti-mouse CD3 Alexa Fluor® 488 only (right).
  • QA17A24_PE-Fire-810_CD103_Antibody_060722
    C57BL/6 splenocytes were stained with anti-mouse CD3 Alexa Fluor® 488 and anti-mouse CD103 recombinant (clone QA17A24) PE/Fire™ 810 or stained with anti-mouse CD3 Alexa Fluor® 488 only (right).
Compare all formats See PE/Fire™ 810 spectral data
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156919 25 µg 222€
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Description

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Mouse
Immunogen
Mouse intestinal intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2924488 (BioLegend Cat. No. 156919)

Antigen Details

Structure
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Interaction
T cells, Dendritic cells, Lymphocytes, Tregs
Ligand/Receptor
E-Cadherin
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Kilshaw PJ and Murant SJ. 1990. Eur. J. Immunol. 20:2201.
  2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
  3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
  4. Sung SS, et al. 2006. J. Immunol. 176:2161.
  5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
  6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.
Gene ID
16407 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/07/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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