PE anti-human Granulysin Antibody

Pricing & Availability
DH2 (See other available formats)
Regulatory Status
Other Names
GNLY, 519, LAG2, NKG5, LAG-2, D2S69E, TLA519
Mouse IgG1, κ
Ave. Rating
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Product Citations
Human peripheral blood lymphocytes surface stained with CD56 APC then intracellularly stained with Granulysin (clone DH2) PE (left) or mouse IgG1, κ PE isotype control (right).
  • DH2_PE_Granulysin_Antibody_091720
    Human peripheral blood lymphocytes surface stained with CD56 APC then intracellularly stained with Granulysin (clone DH2) PE (left) or mouse IgG1, κ PE isotype control (right).
See PE spectral data
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348003 25 tests 168,00€
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348004 100 tests 368,00€
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Granulysin is a member of the saposin-like protein family of lipid binding proteins (SAPLIP). It is expressed by NK cells and cytolytic T lymphocytes (CTL) as 9 kD and 15 kD forms. The 9 kD form is derived from the 15 kD form by cleavage at both amino- and carboxy-termini. Granulysin has broad cytolytic activities against a variety of tumor cells and microbes, including Gram-positive and -negative bacteria, fungi and parasites. It also functions as a chemoattractant for inflammatory cells and activates the cells expression of cytokines and chemokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Recombinant 9 kD granulysin
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone DH2 recognizes both the 9 kD and 15 kD isoforms of Granulysin.1

ELISA Capture: To measure human granulysin, this antibody can be used as a capture antibody in sandwich ELISA format and paired with the biotinylated DH10 antibody (Cat. No. 526103) as the detection antibody. Recombinant Human Granulysin (ELISA Std.) (Cat. No. 552109) can be used as the protein standard.
Flow Cytometry: The fluorochrome-labeled DH2 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify granulysin-producing cells within mixed cell populations.
Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Hanson DA, et al. 1999. Mol. Immunol. 36:413.
  2. Spada FM, et al. 2000. J. Exp. Med. 191:937.
Product Citations
  1. Kalb M, et al. 2012. J Immunol. 188:1583. PubMed
  2. Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
  3. Davis Z, et al. 2016. PLoS Pathog. 12: 1005421. PubMed
AB_10559756 (BioLegend Cat. No. 348003)
AB_2263307 (BioLegend Cat. No. 348004)

Antigen Details

Saposin-like protein family of lipid binding protein, 9 kD and 15 kD

NK cells, cytolytic T cells

Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type
NK cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Molecular Family
Antigen References

1. Krensky AM. 2000. Biochem. Pharmacol. 59:317.
2. Deng A, et al. 2005. J. Immunol. 174:5243.
3. Kaspar AA, et al. 2001. J. Immunol. 167:350.
4. Krensky AM and Clayberger C. 2009. Tissue Antigens 73:193.

Gene ID
10578 View all products for this Gene ID
View information about Granulysin on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 09/17/2020

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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