The LEGENDplex™ Buffer Set A2 includes the following items:
- Setup Beads
- Assay buffer
- Wash buffer
- Plate Sealers
- Matrix B2
LEGENDplex™ Buffer Set A2 is used in Mix and Match Subpanels of COVID-19 Cytokine Storm Panel 1 and Panel 2 with the following products:
741094 LEGENDplex™ COVID-19 Cytokine Storm Panel 1 and 2 Detection Antibodies
741095 LEGENDplex™ COVID-19 Cytokine Storm Panel 1 & 2 Standard
741096 LEGENDplex™ Human IL-6 Capture Bead A3, 14X
741097 LEGENDplex™ Human CCL2 (MCP-1) Bead A4, 14X
741098 LEGENDplex™ Human G-CSF Bead A5, 14X
741099 LEGENDplex™ Human IFN-α2 Bead A6, 14X
741100 LEGENDplex™ Human IL-2 Bead A8, 14X
741101 LEGENDplex™ Human IFN-γ Bead A10, 14X
741102 LEGENDplex™ Human IL-7 Bead B2, 14X
741103 LEGENDplex™ Human IL-1RA Bead B3, 14X
741104 LEGENDplex™ Human CXCL8 (IL-8) Bead B4, 14X
741105 LEGENDplex™ Human TNF-α Bead B5, 14X
741106 LEGENDplex™ Human CXCL10 (IP-10) Bead B6, 14X
741107 LEGENDplex™ Human CCL3 (MIP-1α) Bead B7, 14X
741108 LEGENDplex™ Human IL-10 Bead B9, 14X
741109 LEGENDplex™ Human CCL5 (RANTES) Bead A7, 14X
741113 LEGENDplex™ Human IL-13 Capture Bead A3, 14X
741114 LEGENDplex™ Human GM-CSF Bead A4, 14X
741115 LEGENDplex™ Human IL-1β Bead A5, 14X
741116 LEGENDplex™ Human IL-5 Bead A6, 14X
741117 LEGENDplex™ Human sCD25 (IL-2Ra) Bead A8, 14X
741118 LEGENDplex™ Human IL-4 Bead A10, 14X
741119 LEGENDplex™ Human VEGF Bead B2, 14X
741120 LEGENDplex™ Human IL-17A Bead B3, 14X
741121 LEGENDplex™ Human IL-18 Bead B4, 14X
741122 LEGENDplex™ Human APRIL Bead B5, 14X
741123 LEGENDplex™ Human CCL4 (MIP-1β) Bead B6, 14X
741124 LEGENDplex™ Human IL-15 Bead B7, 14X
741125 LEGENDplex™ Human IL-12p70 Bead B9, 14X
740377 LEGENDplex™ Filter Plate
740379 LEGENDplex™ V-bottom Plate
- Gene ID
- If I don't have a vacuum, how do I remove the liquid from my plate?
If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.