LEGEND MAX™ Human BDNF ELISA Kit

Pricing & Availability
Other Names
Brain-derived neurotrophic factor, ANON2, BLUN2, BDNF, bdnf, BDNF, human bdnf, non-human species bdnf
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446607-Biological-Detectability.jpg
BDNF levels in serum (n = 20, diluted 1:10) and plasma (n = 18) samples from healthy donors were quantified using the LEGEND MAX™ Human BDNF ELISA Kit. Data are represented as the mean + SD.
  • 446607-Biological-Detectability.jpg
    BDNF levels in serum (n = 20, diluted 1:10) and plasma (n = 18) samples from healthy donors were quantified using the LEGEND MAX™ Human BDNF ELISA Kit. Data are represented as the mean + SD.
  • 446607-Standard-Curve.jpg
    Representative LEGEND MAX™ Human BDNF standard curve.
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446607 1 Pre-coated Plate 720€
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Description

Brain-derived neurotrophic factor (BDNF) is the second member of NGF family of neurotrophic factors (neurotrophins), which includes nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammalian species examined to date. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during development. BDNF participates in axonal growth, pathfinding, and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the central nervous system (CNS). High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor. BDNF is also associated with multiple diseases such as Schizophrenia, Rett syndrome, obsessive compulsive disorder, Huntington’s disease, eating disorders, depression, dementia and Alzheimer’s disease. For example, BDNF level is decreased in Alzheimer’s disease patients’ serum samples.

The BioLegend LEGEND MAX™ Human BDNF Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human BDNF capture antibody. The detection antibody is a biotinylated mouse monoclonal anti-human BDNF antibody. This kit is specifically designed for the accurate quantitation of the free-form human BDNF from cell culture supernatant, serum, plasma, and other biological fluids. It has been analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human BDNF Pre-coated 96-well Strip Microplate
  • Human BDNF Dectection Antibody
  • Human BDNF Standard
  • Avidin-HRP
  • Assay Buffer A
  • Assay Diluent C
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Reactivity
Human
Sensitivity
27.5 pg/mL
Standard Range
156.3-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Gene ID
627 View all products for this Gene ID
UniProt
View information about BDNF on UniProt.org

Related FAQs

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

Go To Top Version: 1    Revision Date: 09/28/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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