- MP4-25D2 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.Product Details
- Verified Reactivity
- Human, Cross-Reactivity: Swine (Pig, Porcine), Rhesus
- Antibody Type
- Host Species
- CHO-expressed, recombinant human IL-4
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
ELISA Detection1,3 or ELISPOT Detection4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.
(PubMed link indicates BioLegend citation)
- Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
- Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
- Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
- Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
- Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
- Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
- Raqib R, et al. 1995. Infect. Immun. 63:289.
- Andersson J, et al. 1994. Immunology 83:16.
- Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
- Kubota M, et al. 1997. J. Immunol. 158:5321.
- Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
- Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed
- Product Citations
AB_2562311 (BioLegend Cat. No. 500827)
AB_2563879 (BioLegend Cat. No. 500828)
- Cytokine; 15-19 kD (Mammalian)
- Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
- Cell Sources
- Mast cells, T cells, bone marrow stromal cells
- Cell Targets
- B cells, T cells, monocytes, endothelial cells, fibroblasts
- Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
- Cell Type
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.
- Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
- Gene ID
- 3565 View all products for this Gene ID
- View information about IL-4 on UniProt.org
|PE/Dazzle™ 594 anti-human IL-4||MP4-25D2||ICFC|
|APC/Cyanine7 anti-human IL-4||MP4-25D2||ICFC|
|Brilliant Violet 510™ anti-human IL-4||MP4-25D2||ICFC|
|Ultra-LEAF™ Purified anti-human IL-4||MP4-25D2||ELISA, CyTOF®, Neut, ICFC, IHC|
|Alexa Fluor® 488 anti-human IL-4||MP4-25D2||ICFC|
|Alexa Fluor® 647 anti-human IL-4||MP4-25D2||ICFC|
|PerCP/Cyanine5.5 anti-human IL-4||MP4-25D2||ICFC|
|PE/Cyanine7 anti-human IL-4||MP4-25D2||ICFC|
|Brilliant Violet 421™ anti-human IL-4||MP4-25D2||ICFC|
|Brilliant Violet 605™ anti-human IL-4||MP4-25D2||ICFC|
|Purified anti-human IL-4 (Maxpar® Ready)||MP4-25D2||ELISA, CyTOF®|
|APC anti-human IL-4||MP4-25D2||ICFC|
|Biotin anti-human IL-4||MP4-25D2||ELISA Detection, ELISPOT Detection, ICFC|
|FITC anti-human IL-4||MP4-25D2||ICFC|
|PE anti-human IL-4||MP4-25D2||ICFC|
|Purified anti-human IL-4||MP4-25D2||ELISA, CyTOF®, Neut, ICFC, IHC|
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE/Dazzle™ 594 anti-human IL-4
APC/Cyanine7 anti-human IL-4
Brilliant Violet 510™ anti-human IL-4
Ultra-LEAF™ Purified anti-human IL-4
Alexa Fluor® 488 anti-human IL-4
Alexa Fluor® 647 anti-human IL-4
PerCP/Cyanine5.5 anti-human IL-4
PE/Cyanine7 anti-human IL-4
Brilliant Violet 421™ anti-human IL-4
Brilliant Violet 605™ anti-human IL-4
Purified anti-human IL-4 (Maxpar® Ready)
APC anti-human IL-4
Biotin anti-human IL-4
FITC anti-human IL-4
PE anti-human IL-4
Purified anti-human IL-4