- 2D1 (See other available formats)
- Regulatory Status
- Other Names
- Leukocyte Common Antigen (LCA), T200
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.Product Details
- Antibody Type
- Host Species
- Human PBMC
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
(PubMed link indicates BioLegend citation)
- Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
- Csiba A, et al. 1984. Br. J. Cancer 50:699.
- Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
- Lee MS, et al. 2004. Int. Immunol. 16:1109.
- Product Citations
AB_2566373 (BioLegend Cat. No. 368513)
AB_2566374 (BioLegend Cat. No. 368514)
- Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
Hematopoietic cells, except circulating erythrocytes or platelets.
- Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
- Galectin-1, CD2, CD3, and CD4.
- Cell Type
- B cells, Dendritic cells, Neutrophils
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Protein Kinases/Phosphatase, TCRs
- Antigen References
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- View information about CD45 on UniProt.org
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