Alexa Fluor® 488 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_A488_CD3_Antibody_4_092121
Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3 Antibody (clone 17A2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
Watch the video.
  • 17A2_A488_CD3_Antibody_4_092121
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse CD3 Antibody (clone 17A2) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 594 at 5 µg/mL (blue), and anti-mouse CD68 Antibody (clone FA-11) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
    Watch the video.
  • 17A2_Alexa488_071805
    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 488
  • 17A2_Alexa488_CD3_IHC-F_120816
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL CD3 (Clone 17A2) Alexa Fluor® 488 (green) and B220 (Clone RA3-6B2) Brilliant Violet 421™ (blue), overnight at 4°C. The image was captured by 10X objective.
  • 3_Still-image_0316-5_20X-1_1
    Formalin-fixed, 300 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with CD3 (clone 17A2) Alexa Fluor® 488 (red), CD21/35 (CR2/CR1)(clone 7E9) Alexa Fluor® 594 (green), and CD45R/B220 (clone RA3-6B2) Alexa Fluor® 647 (blue) all at 5 µg/mL, optically cleared, then analyzed at 215 μm imaging depth on a confocal microscope.
See Alexa Fluor® 488 spectral data See high resolution IHC-F data...
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100212 25 µg 136,00€
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100210 100 µg 312,00€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Product Citations
  1. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  2. Zhao L,et al. 2017. Nat Commun. . 10.1038/s41467-017-01181-4. PubMed
  3. Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
  4. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  5. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  6. Karandikar SH, et al. 2019. Anal Chem. 91:3405. PubMed
  7. Broderick L, et al. 2019. Nat Commun. 10:3644. PubMed
  8. Karandikar SH, et al. 2019. JCI Insight. 5. PubMed
  9. Schneider C, et al. 2018. Cell. 174:271. PubMed
  10. Kodumudi KN, et al. 2019. Front Immunol. 10:1939. PubMed
  11. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  12. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  13. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  14. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  15. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  16. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  17. Lee-Sayer SSM, et al. 2018. Eur J Immunol. 48:803. PubMed
  18. Pietronigro E, et al. 2019. Sci Rep. 9:12055. PubMed
  19. Cheng Q, et al. 2018. PLoS Pathog. 14:e1007440. PubMed
  20. Xiao S, et al. 2007. J Exp Med. 204:1691. PubMed
  21. Wan W, et al. 2013. Cardiovasc Res. 97:580. PubMed
  22. Langhauser F, et al. 2014. Stroke. 45:1799. PubMed
  23. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
  24. Hsieh C, et al. 2014. J Immunol. 193:3693. PubMed
  25. Hanihara-Tatsuzawa F, et al. 2014. J Biol Chem. 389:30925. PubMed
  26. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
  27. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  28. Amor C, et al. 2020. Nature. 583:127. PubMed
  29. Counoupas C, et al. 2020. NPJ Vaccines. 0.28125. PubMed
  30. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  31. Upadhyaya P, et al. 2021. J Immunother Cancer. 9:00. PubMed
  32. Wang C, et al. 2021. Cell Stem Cell. . PubMed
  33. Wong HS, et al. 2021. Cell. . PubMed
RRID
AB_493530 (BioLegend Cat. No. 100212)
AB_389301 (BioLegend Cat. No. 100210)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 09/21/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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