7-AAD Viability Staining Solution

Pricing & Availability
Regulatory Status
RUO
Other Names
Viability dye, dead cell exclusion dye, cell viability dye
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
420403 200 tests 40,00€
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420404 500 tests 80,00€
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Description

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation
CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Concentration
50 µg/ml
Storage & Handling
Protect from light. Store between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. The analysis of the cells should be done as soon as possible after the incubation with the dye.

We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.

 

Excitation Laser
Blue Laser (488 nm)
Application Notes

7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
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Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience
Antigen References

1. Schmid I, et al. 1992. Cytometry. 13:204

Gene ID
NA

Related FAQs

Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?

These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.

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LEGEND MAX™ Mouse GM-CSF ELISA Kit ELISA
LEGEND MAX™ Human GM-CSF ELISA Kit ELISA
LEGEND MAX™ Human IL-2 ELISA Kit ELISA
LEGEND MAX™ Human IL-8 ELISA Kit ELISA
LEGEND MAX™ Mouse IL-6 ELISA Kit ELISA
LEGEND MAX™ Mouse IL-2 ELISA Kit ELISA
LEGEND MAX™ Mouse IFN-γ ELISA Kit ELISA
LEGEND MAX™ Human IL-10 ELISA Kit ELISA
LEGEND MAX™ Human IL-6 ELISA Kit ELISA
LEGEND MAX™ Human TNF-α ELISA Kit ELISA
LEGEND MAX™ Human IFN-γ ELISA Kit ELISA
LEGEND MAX™ Human IL-28A ELISA Kit with Pre-coated Plates ELISA
LEGEND MAX™ Human IL-4 ELISA Kit ELISA
LEGEND MAX™ Human IL-15 ELISA Kit ELISA
LEGEND MAX™ Mouse IL-12 (p70) ELISA Kit ELISA
LEGEND MAX™ Mouse IL-4 ELISA Kit ELISA
LEGEND MAX™ Human IL-22 ELISA Kit ELISA
Recombinant Human IL-17A/F Heterodimer (carrier-free) BA
Recombinant Rat TNF-α (carrier-free) BA
Recombinant Human CCL5 (RANTES) (Animal-Free) BA
Recombinant Human CCL5 (RANTES) (carrier-free) BA
Recombinant Human LT-α (TNF-β) ELISA, BA
Recombinant Mouse IFN-β1 (carrier-free) BA
Recombinant Mouse VEGF-120 (carrier-free) BA
Recombinant Mouse IL-10 (ELISA Std.) ELISA
Recombinant Human PDGF-BB (carrier-free) BA
Recombinant Human CCL2 (MCP-1) (carrier-free) BA
Recombinant Mouse M-CSF (carrier-free) BA
Recombinant Human CXCL12 (SDF-1α) (carrier-free) BA
Recombinant Mouse IL-3 (carrier-free) BA
Recombinant Human GM-CSF (carrier-free) BA
Recombinant Mouse SCF (carrier-free) BA
LEGEND MAX™ Mouse IL-22 ELISA Kit ELISA
LEGEND MAX™ Human IL-33 ELISA Kit ELISA
LEGEND MAX™ Human IL-17F ELISA Kit ELISA
LEGEND MAX™ Mouse IL-34 ELISA Kit ELISA
FOXP3 Fix/Perm Buffer (4x) ICFC
Recombinant Human IL-1α (ELISA Std.) ELISA
Recombinant Human IL-17F (carrier-free) BA
Recombinant Human IL-10 (ELISA Std.) ELISA
Recombinant Human IL-13 (ELISA Std.) ELISA
Recombinant Human IL-21 (ELISA Std.) ELISA
Recombinant Human IL-5 (ELISA Std.) ELISA
Recombinant Human IL-12/IL-23 (p40) (ELISA Std.) ELISA
Recombinant Human GM-CSF (ELISA Std.) ELISA
Recombinant Mouse IL-1β (ELISA Std.) ELISA
Recombinant Mouse IL-4 (ELISA Std.) ELISA
Recombinant Mouse IL-17A (ELISA Std.) ELISA
Recombinant Mouse MCP-1 (ELISA Std.) ELISA
Recombinant Mouse IL-12/IL-23 (p40) (ELISA Std.) ELISA
Recombinant Mouse IL-12 (p70) (ELISA Std.) ELISA
Recombinant Human IL-1β (ELISA Std.) ELISA
Human TruStain FcX™ (Fc Receptor Blocking Solution) FC, ICC, ICFC
Recombinant Mouse IL-12 (p70) (carrier-free) BA
Recombinant Mouse IL-17F (carrier-free) BA
Recombinant Human IL-12 (p70) (carrier-free) BA
Recombinant Human EGF (Animal-Free) BA
Recombinant Human IFN-γ (Animal-Free) BA
LEGEND MAX™ Human IL-12 (p70) ELISA Kit ELISA
LEGEND MAX™ Human MRP8/14 (Calprotectin) ELISA Kit ELISA
Recombinant Human TWEAK (CD255) (carrier-free) BA
Recombinant Mouse IL-15 (carrier-free) BA
Recombinant Human IL-4 (carrier-free) BA
LEGEND MAX™ Human FGL2 ELISA Kit ELISA
LEGEND MAX™ Human IL-13 ELISA Kit ELISA
LEGEND MAX™ Human IL-12/IL-23 (p40) ELISA Kit ELISA
LEGEND MAX™ Mouse IL-17F ELISA Kit ELISA
LEGEND MAX™ Mouse IL-33 ELISA Kit ELISA
Recombinant Human IL-23 (carrier-free) BA
Recombinant Human IL-8 (carrier-free) BA
Recombinant Human IL-2 (carrier-free) BA
Recombinant Mouse IL-27 (carrier-free) BA
Recombinant Mouse IL-12 p40 Homodimer (carrier-free) BA
ELISA MAX™ Deluxe Set Mouse IL-22 ELISA
DyLight™ 488 Streptavidin FC, ICC, IHC-F, IHC-P
DyLight™ 649 Streptavidin FC, ICC, IHC-F, IHC-P
LEGEND MAX™ Rat IL-6 ELISA Kit ELISA
Recombinant Mouse EGF (Animal-Free) BA
Recombinant Mouse FGF-basic (Animal-Free) BA
Recombinant Mouse G-CSF (Animal-Free) BA
Recombinant Human VEGF-165 (Animal-Free) BA
Recombinant Human TNF-α (Animal-Free) BA
Recombinant Human TRANCE (RANKL) (Animal-Free) BA
Recombinant Human IL-27 (carrier-free) BA
LEGEND MAX™ Human IL-1β ELISA Kit ELISA
Recombinant Mouse IL-34 (carrier-free) BA
Recombinant Human TGF-α (Animal-Free) BA
Recombinant Human Thrombopoietin (TPO) (Animal-Free) BA
Recombinant Human GM-CSF (Animal-Free) BA
LEGEND MAX™ Mouse IL-17A/F ELISA Kit ELISA
LEGEND MAX™ Rat IL-17A ELISA Kit ELISA
Recombinant Human Oncostatin M (Animal-Free) BA
Recombinant Human PDGF-AA (Animal-Free) BA
ELISA MAX™ Deluxe Set Rat TNF-a ELISA
ELISA MAX™ Deluxe Set Rat IL-17A ELISA
Brilliant Violet 421™ Streptavidin FC, ICFC, ICC, IHC-F, IHC-P
Recombinant Mouse TACI-Fc Chimera (carrier-free) BA
FOXP3 Perm Buffer (10x) ICFC
Recombinant Mouse IL-7 (carrier-free) BA
Recombinant Human IL-34 (carrier free) BA
Recombinant Human M-CSF (Animal-Free) BA
Recombinant Human KGF (FGF-7) (Animal-Free) BA
Recombinant Human LIF (Animal-Free) BA
RBC Lysis/Fixation Solution (10X) FC
Recombinant Mouse CXCL9 (MIG) (carrier-free) BA
LEGEND MAX™ Rat IFN-γ ELISA Kit ELISA
LEGEND MAX™ Mouse IL-27 Heterodimer ELISA Kit ELISA
LEGEND MAX™ Human Active MIF ELISA Kit ELISA
LEGEND MAX™ Human IL-17A/F ELISA Kit ELISA
Brilliant Violet 570™ Streptavidin FC, ICFC
Recombinant Human CXCL9 (MIG) (carrier-free) BA
Recombinant Human IL-3 (carrier-free) BA
ELISA MAX™ Deluxe Set Human Mesothelin ELISA
Recombinant Human IL-36γ (Animal-Free) BA
Recombinant Human CXCL1 (GRO-α) (carrier-free) BA
ELISA MAX™ Deluxe Set Human IL-17A ELISA
LEGEND MAX™ Human IL-17A ELISA Kit ELISA
Recombinant Mouse IL-21 (carrier-free) BA
Recombinant Mouse CXCL5 (LIX) (carrier-free) BA
Recombinant Human CXCL5 (ENA-78) (carrier-free) BA
Recombinant Human CXCL10 (IP-10) (carrier-free) BA
Recombinant Human IL-12 (p70) (ELISA Std.) ELISA
Recombinant Human IL-22 (Animal-Free) BA
Recombinant Human IL-33 (Animal-Free) BA
Recombinant Mouse CXCL10 (IP-10) (carrier-free) BA
Recombinant Mouse CXCL1 (KC) (carrier-free) BA
Recombinant Human SCF (carrier-free) BA
Recombinant Mouse G-CSF (carrier-free) BA
Recombinant Human CXCL13 (carrier-free) BA
ELISA MAX™ Deluxe Set Human MCP-1/CCL2 ELISA
Recombinant Human M-CSF (carrier-free) BA
Recombinant Human CXCL11 (ITAC) (carrier-free) BA
Recombinant Mouse CXCL11 (ITAC) (carrier-free) BA
Recombinant Mouse CCL2 (MCP-1) (carrier-free) BA
Recombinant Human G-CSF (carrier-free) BA
Recombinant Mouse CXCL12 (SDF-1α) (carrier-free) BA
Recombinant Mouse CCL17 (TARC) (carrier-free) BA
Recombinant Mouse IL-5 (carrier-free) BA
LEGEND MAX™ Mouse Latent TGF-β ELISA Kit ELISA
LEGEND MAX™ Human Granzyme B ELISA Kit ELISA
Recombinant Human CCL8 (MCP-2) (carrier-free) BA
Recombinant Mouse CCL8 (MCP-2) (carrier-free) BA
Brilliant Violet 605™ Streptavidin FC, ICFC
Brilliant Violet 650™ Streptavidin FC, ICFC
Recombinant Mouse ICAM-1-Fc Chimera (carrier-free) BA
Recombinant Human IL-33 (carrier-free) BA
Recombinant Human IL-7 (carrier-free) BA
Recombinant Human CXCL2 (Groβ) (carrier-free) BA
Recombinant Human CCL19 (MIP-3β) (carrier-free) BA
Recombinant Human CCL21 (6CKine) (carrier-free) BA
Recombinant Mouse CCL20 (MIP-3α) (carrier-free) BA
LEGEND MAX™ Mouse IFN-β ELISA Kit ELISA
LEGEND MAX™ Human APRIL/TNFSF13 ELISA Kit ELISA
LEGEND MAX™ Mouse IL-10 ELISA Kit ELISA
DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) ICFC, ICC
Rainbow Fluorescent Particles, 1 peak (3.0-3.4 µm) - Mid Range Intensity FC
Rainbow Calibration Particles, 8 peaks (3.0-3.4 µm) FC
Rainbow Calibration Particles, 6 peaks (3.0-3.4 µm) FC
Rainbow Fluorescent Particles, 1 peak (3.0-3.4 µm) - Bright Intensity FC
Cyto-Last™ Buffer ICFC
Recombinant Human TSLP (carrier-free) BA
Recombinant Mouse CXCL2 (MIP-2) (carrier-free) BA
Recombinant Human CCL1 (I-309) (carrier-free) BA
ELISA MAX™ Deluxe Set Mouse IL-10 ELISA
ELISA MAX™ Standard Set Mouse IL-10 ELISA
Recombinant Mouse CCL11 (Eotaxin) (carrier-free) BA
Recombinant Human CCL11 (Eotaxin) (carrier-free) BA
Recombinant Mouse VEGF-164 (carrier-free) BA
Recombinant Human VEGF-121 (carrier-free) BA
Recombinant Human TGF-β2 (carrier-free) BA
LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit ELISA
LEGEND MAX™ Human IL-34 ELISA Kit ELISA
ELISA MAX™ Deluxe Set Human IL-34 ELISA
Brilliant Violet 510™ Streptavidin FC, ICFC
APC Annexin V FC
PE Annexin V FC
Recombinant Human CX3CL1 (Fractalkine) (carrier-free) BA
Recombinant Mouse CX3CL1 (Fractalkine) (carrier-free) BA
Recombinant Human CCL27 (CTACK) (carrier-free) BA
Recombinant Human CCL22 (MDC) (carrier-free) BA
FITC Annexin V Apoptosis Detection Kit with PI FC
Recombinant Mouse CCL24 (Eotaxin-2) (carrier-free) BA
Recombinant Human CCL24 (Eotaxin-2) (carrier-free) BA
Recombinant Human VEGF-165 (carrier-free) BA
Recombinant Human CCL28 (MEC) (carrier-free) BA
Recombinant Mouse CCL28 (MEC) (carrier-free) BA
Recombinant Mouse CCL1 (I-309) (carrier-free) BA
LEGEND MAX™ Human Myeloperoxidase ELISA Kit ELISA
Recombinant Human CCL20 (MIP-3α) (carrier-free) BA
FITC Annexin V Apoptosis Detection Kit with 7-AAD FC
Recombinant Mouse IL-33 (ELISA Std.) ELISA
Recombinant Human IL-22 (ELISA Std.) ELISA
Recombinant Human CXCL17 (VCC-1) (carrier-free) BA
Recombinant Mouse CXCL17 (VCC-1) (carrier-free) BA
Recombinant Human EGF (carrier-free) BA
Recombinant Mouse EGF (carrier-free) BA
Recombinant Mouse IL-6 (Animal-Free) BA
Recombinant Human TGF-β3 (carrier-free) BA
Recombinant Human IL-11 (carrier-free) BA
Recombinant Mouse CCL7 (MCP-3) (carrier-free) BA
Recombinant Mouse CCL9 (MIP-1γ) (carrier-free) BA
Recombinant Mouse CCL21 (6CKine) (carrier-free) BA
Recombinant Mouse Thrombopoietin (TPO) (Animal-Free) BA
Recombinant Mouse IL-3 (Animal-Free) BA
Recombinant Mouse IL-4 (Animal-Free) BA
Recombinant Human IL-21 (carrier-free) BA
Recombinant Mouse CXCL13 (carrier-free) BA
LEGEND MAX™ Human sICAM-1/CD54 ELISA Kit with Pre-coated Plates ELISA
Stop Solution for TMB Substrate ELISA
Zombie Aqua™ Fixable Viability Kit FC, ICFC, ICC
LEGEND MAX™ Human CXCL10 (IP-10) ELISA Kit ELISA
Recombinant Mouse CXCL7 (NAP-2) (carrier-free) BA
Recombinant Mouse CXCL3 (carrier-free) BA
Recombinant Mouse CCL25 (TECK) (carrier-free) BA
Recombinant Human CCL25 (TECK) (carrier-free) BA
Recombinant Human CCL23 (MPIF-1) (Arg46-Asn120) (carrier-free) BA
Recombinant Human Erythropoietin (EPO) (carrier-free) BA
Recombinant Mouse IL-25 (IL-17E) (insect expressed, carrier-free) BA
Recombinant Human FASL (TNFSF6) (carrier-free) BA
ELISA MAX™ Deluxe Set Human CXCL10 (IP-10) ELISA
Recombinant Mouse CXCL14 (BRAK) (carrier-free) BA
Recombinant Human CXCL14 (BRAK) (carrier-free) BA
Recombinant Mouse Erythropoietin (EPO) (carrier-free) BA
Recombinant Human CXCL12 (SDF-1β) (carrier free) BA
Recombinant Mouse CCL19 (MIP-3β) (carrier-free) BA
Recombinant Mouse CCL12 (MCP-5) (carrier-free) BA
Zombie NIR™ Fixable Viability Kit FC, ICFC
Recombinant Human VEGF-C (carrier-free) BA
Recombinant Mouse NGAL (Lipocalin-2) (carrier-free) BA
Recombinant Human NGAL (Lipocalin-2) (carrier-free) BA
Recombinant Mouse IGF-II (carrier-free) BA
Purified Annexin V FC
Recombinant Mouse CXCL12 (SDF-1β) (carrier-free) BA
Recombinant Human TGF-α (carrier-free) BA
Recombinant Rat CCL20 (MIP-3α) (carrier-free) BA
LEGEND MAX™ Human CCL17 (TARC) ELISA Kit ELISA
LEGEND MAX™ Human CCL5 (RANTES) ELISA Kit ELISA
Recombinant Mouse CXCL4 (PF-4) (carrier-free) BA
Accutase® Cell Detachment Solution FC
Recombinant Human IGF-II (carrier-free) BA
Recombinant Human IGF-I (carrier-free) BA
Recombinant Human TRANCE (RANKL) (carrier-free) BA
Recombinant Mouse BAFF (carrier-free) BA
Recombinant Human APRIL (TNFSF13) (ELISA Std.) ELISA
Recombinant Human IL-15 (ELISA Std.) ELISA
Recombinant Mouse IGF-I (carrier-free) BA
Recombinant Human sTNF-RII (TNFRSF1B) (carrier-free) BA
ELISA MAX™ Deluxe Set Human CCL17 (TARC) ELISA
Recombinant Mouse CTLA-4-Fc Chimera (carrier-free) BA
Recombinant Human CD40L (TNFSF5) (carrier-free) BA
Recombinant Human CTLA-4-Fc Chimera (carrier-free) BA
Recombinant Mouse TNF-α (Animal-Free) BA
Recombinant Human Activin A (carrier-free) BA
Recombinant Human FGF-4 (carrier-free) BA
Recombinant Human IL-18 (carrier-free) BA
Recombinant Rat GM-CSF (carrier-free) BA
Recombinant Human IFN-α2 (carrier-free) BA
ELISA MAX™ Deluxe Set Human CCL5 (RANTES) ELISA
ELISA MAX™ Deluxe Set Human CXCL5 ELISA
LEGEND MAX™ Mouse IL-35 Heterodimer ELISA Kit ELISA
ELISA MAX™ Deluxe Set Human CCL28 ELISA
Recombinant Rat Erythropoietin (EPO) (carrier-free) BA
Recombinant Human PCSK9 (carrier-free) BA
Recombinant Human CCL17 (TARC) (ELISA Std.) ELISA
Recombinant Human TIMP-1 (carrier-free) BA
Recombinant Human Arginase I (carrier-free) BA
Recombinant Human MMP-1 (carrier-free) BA
Recombinant Human CD14 (carrier-free) BA
Recombinant Human CTLA-4 (ELISA Std.) ELISA
Recombinant Mouse IL-34 (ELISA Std.) ELISA
Recombinant Human IL-34 (ELISA Std.) ELISA
Recombinant Human Latent TGF-β (ELISA Std.) ELISA
Recombinant Human Mesothelin-Fc Chimera (carrier-free) BA
Recombinant Mouse Thrombopoietin (TPO) (carrier-free) BA
Recombinant Human TIMP-2 (carrier-free) BA
Recombinant Mouse TIMP-1 (carrier-free) BA
Brilliant Violet 421™ Annexin V FC
Alexa Fluor® 488 Streptavidin FC, ICFC
Alexa Fluor® 647 Streptavidin FC, ICFC
Recombinant Mouse CCL3 (MIP-1α) (carrier-free) BA
Recombinant Human LIF (carrier-free) BA
Recombinant Mouse Mesothelin (carrier-free) BA
Recombinant Rat IFN-γ (ELISA Std.) ELISA
Recombinant Mouse IL-17A/F Heterodimer (ELISA Std.) ELISA
Recombinant Human TGF-β1 (ELISA Std.) ELISA
Zombie Violet™ Fixable Viability Kit FC, ICFC, ICC
Zombie Green™ Fixable Viability Kit FC, ICFC, ICC
Zombie Red™ Fixable Viability Kit FC, ICFC, ICC
Zombie UV™ Fixable Viability Kit FC, ICFC
CFSE Cell Division Tracker Kit ICFC, in-vivo Cell Tracking
LEGEND MAX™ Human Adiponectin (Acrp30) ELISA Kit ELISA
Bromodeoxyuridine (BrdU) FC, ICFC
Recombinant Mouse CCL5 (RANTES) (carrier-free) BA
Recombinant Mouse IFN-β (ELISA Std.) ELISA
Recombinant Human IL-9 (carrier-free) BA
Recombinant Mouse Latent TGF-β (ELISA Std.) ELISA
Recombinant Human MMP-3 (carrier-free) BA
APC Streptavidin (High Concentration) FC, ICFC
Recombinant Human CCL2 (MCP-1) (Animal-Free) BA
Recombinant Human VEGFR2-Fc Chimera (carrier-free) BA
Recombinant Human NT-4 (Animal-Free) BA
Cell Activation Cocktail (without Brefeldin A) Activ, FC, ICFC
Recombinant Human RBP4 (carrier-free) BA
Recombinant Rat IFN-γ (carrier-free) BA
Recombinant Human ICOS-Fc Chimera (carrier-free) BA
Cell Activation Cocktail (with Brefeldin A) Activ, FC, ICFC
Recombinant Mouse ICOS-Fc Chimera (carrier-free) BA
Recombinant Mouse VEGF-164 (Animal-Free) BA
Recombinant Human NT-3 (carrier-free) BA
Recombinant Human NT-4 (carrier-free) BA
Recombinant Human FGF-1-acidic (carrier-free) BA
Nunc™ MaxiSorp™ ELISA Plates, Uncoated ELISA
Plate Sealers ELISA
DRAQ7™ FC, ICFC
Recombinant Human WISP-1 (carrier-free) BA
Recombinant Human WNT-7a (carrier-free) BA
Recombinant Human BMP-4 (carrier-free) BA
Go To Top Version: 2    Revision Date: 05/21/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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