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Ultra-LEAF™ Purified anti-human CXCL11 (ITAC) Antibody

Pricing & Availability
Clone
A15129H (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-inducible T-cell alpha chemoattractant (ITAC), Small inducible cytokine, subfamily B, member 9B (SCYB9B), Small inducible cytokine, subfamily B, member 11 (SCYB11)
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A16031D_ULEAF_Human_CXCL11_ITAC_Antibody_060121
Recombinant human CXCL11 (ITAC) (Cat. No. 574904) (black circles) induces the chemotaxis of mouse Baf3-mCXCR3 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CXCL11 (ITAC) antibody (clone A15129H) (purple squares) neutralizes the chemotaxis of recombinant human CXCL11 (ITAC) at 15 ng/mL on mouse Baf3-mCXCR3 cells in a dose-dependent manner whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400264) (gray triangles) does not have the effect. ND50 range: 0.01 - 0.1 µg/mL.
  • A16031D_ULEAF_Human_CXCL11_ITAC_Antibody_060121
    Recombinant human CXCL11 (ITAC) (Cat. No. 574904) (black circles) induces the chemotaxis of mouse Baf3-mCXCR3 transfectant chemoattractant cells. Ultra-LEAF™ purified anti-human CXCL11 (ITAC) antibody (clone A15129H) (purple squares) neutralizes the chemotaxis of recombinant human CXCL11 (ITAC) at 15 ng/mL on mouse Baf3-mCXCR3 cells in a dose-dependent manner whereas the Ultra-LEAF™ purified mouse IgG2a, κ isotype ctrl antibody (Cat. No. 400264) (gray triangles) does not have the effect. ND50 range: 0.01 - 0.1 µg/mL.
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945403 100 µg 287€
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945404 1 mg 964€
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Description

CXCL11 is a CXC chemokine lacking the ELR (Glutamate-Leucine-Arginine) tripeptide motif, which is functionally and structurally related to CXCL9 and CXCL10. CXCL11, together with CXCL9 and CXCL10, specifically binds to the G protein-coupled receptor CXCR3 and is involved in chemotaxis of immune cells and angiogenesis. CXCL11 expression is upregulated in response to interferons (IFNs). Expression of both CXCR3 and CXCL11 by the Th1-associated cytokine IFN-γ creates an amplification loop of cell-mediated immune response between Th1 cells. CXCR7 has been identified as an alternate receptor for CXCL11 and is involved in growth, survival, and adhesion of tumor cells. CXCL11 induces calcium release in activated T-cells and may play an important role in CNS diseases which involve T-cell recruitment.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CXCL11
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of chemotaxis induced by recombinant human CXCL11 (ITAC) (Cat. No. 574904) at 15 ng/mL on mouse Baf3-mCXCR3 transfectant chemoattractant cells. ND50 range: 0.01 - 0.1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2892512 (BioLegend Cat. No. 945403)
AB_2892512 (BioLegend Cat. No. 945404)

Antigen Details

Structure
Chemokine
Distribution

T lymphocytes, keratinocytes, monocytes, macrophages, fibroblasts, and endothelial cells

Function
Chemoattractant for activated T lymphocytes and NK cells, stimulation of IFN-γ expression, inhibition of angiogenesis
Interaction
Activated T cells, Th1 cells, regulatory T cells, NK cells, endothelial cells
Ligand/Receptor
CXCR3-A, CXCR3-B, CXCR7
Antigen References

1.Widney DP, et al. 2000. J. Immunol. 164:6322.
2. Meyer M, et al. 2000. Cytogenet. Cell Genet. 88:278.
3. Loetscher P, et al. 2001. J. Biol. Chem. 276(5):2986-91.

Gene ID
6373 View all products for this Gene ID
UniProt
View information about CXCL11 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 06/01/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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