Ultra-LEAF™ Purified anti-human CCL3 (MIP-1α) Antibody

Pricing & Availability
Clone
W16009B (See other available formats)
Regulatory Status
RUO
Other Names
Macrophage inflammatory protein 1-alpha (MIP-1-alpha), LD78-alpha, SCYA3, G0S19-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16009B_ULEAF_CCL3_MIP-1alpha_Antibody_072821.png
Recombinant human CCL3 (Black Circles, Cat. No. 759506) induces the chemotaxis of mouse Baf3-hCCR5 transfectants chemoattractant cells. Anti-human CCL3 antibody (clone W16009B, Purple Squares) neutralizes the chemotactic activity of recombinant human CCL3 (10 ng/mL) on Baf3-hCCR5 cells in a dose-dependent manner. The ND50 range: 2 - 8 µg/mL.
  • W16009B_ULEAF_CCL3_MIP-1alpha_Antibody_072821.png
    Recombinant human CCL3 (Black Circles, Cat. No. 759506) induces the chemotaxis of mouse Baf3-hCCR5 transfectants chemoattractant cells. Anti-human CCL3 antibody (clone W16009B, Purple Squares) neutralizes the chemotactic activity of recombinant human CCL3 (10 ng/mL) on Baf3-hCCR5 cells in a dose-dependent manner. The ND50 range: 2 - 8 µg/mL.
Cat # Size Price Quantity Avail. Save
934603 100 µg 196,00€
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934604 1 mg 520,00€
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Description

CCL3 is a chemokine belonging to the CC chemokine family. CCL3 is involved in activation and recruitment of lymphocytes, monocytes, and granulocytes during acute inflammation. In addition to regulating leukocyte migration, CCL3 enhances IFN-γ secretion from activated T cells and thus induces a Th1 response. Moreover, CCL3 has been reported to be involved in susceptibility to HIV infection and disease progression of AIDS. Human CCL3 is also demonstrated to inhibit proliferation of hematopoietic progenitor stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human CCL3
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralization of the chemotaxis induced by recombinant human CCL3 (40 ng/mL) on mouse Baf3-hCCR5 transfectants chemoattractant cells. ND50 range: 2 - 8 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894523 (BioLegend Cat. No. 934603)
AB_2894523 (BioLegend Cat. No. 934604)

Antigen Details

Structure
CC chemokine.
Distribution

T lymphocytes, B lymphocytes, monocytes, macrophage, dendritic cells, NK cells, neutrophils, basophils, eosinophils, platelets, fibroblasts, osteoblasts, astrocytes, vascular smooth muscle cells, fetal microglia, and epithelial cells.

Function
The CCL3 chemokine is a chemoattractant which recruits and activates leukocytes to sites of infection or inflammation, resulting in an enhanced local inflammatory response.
Interaction
T lymphocytes, monocytes, NK cells, immature dendritic cells, neutrophils, basophils, eosinophils, platelets, and coronary epithelial cells.
Ligand/Receptor
CCR1, CCR4, and CCR5.
Cell Type
Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. Menten P, et al. 2002. Cytokine Growth Factor Rev. 13:455.
2. Blum S, et al. 1990. DNA Cell Biol. 9:589.
3. Czaplewski LG, et al. 1999. J Biol Chem. 274:16077.
4. Paximadis M, et al. 2009. Int J Immunogenet. 36:21.
5. Cocchi F, et al. 1995. Science. 270:1811.
6. Meddows-Taylor S, et al. 2006. J Gen Virol. 87:2055.

Gene ID
6348 View all products for this Gene ID
UniProt
View information about CCL3 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 07/28/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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