Ultra-LEAF™ Purified anti-GDNF Antibody

Pricing & Availability
Clone
A16099G (See other available formats)
Other Names
Glial cell line-derived neurotrophic factor, Astrocyte-derived trophic factor (ATF), ATF1, ATF2, HSCR3, HFB1-GDNF
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A16099G_ULEAF_GDNF_Antibody_1_121319
Recombinant human GFRα1 (black circles) binds to immobilized recombinant human GDNF (Cat. No. 760402). Anti-human GDNF antibody (purple squares, clone A16099G) inhibits the binding in a dose-dependent manner while the isotype control (grey triangles, Cat. No. 401215) does not have the effect. This antibody blocks the binding of 0.1 µg/mL recombinant human GFRα1 protein to 0.5 µg/mL of immobilized recombinant human GDNF with an ND50 = 0.05 - 0.4 µg/mL.
  • A16099G_ULEAF_GDNF_Antibody_1_121319
    Recombinant human GFRα1 (black circles) binds to immobilized recombinant human GDNF (Cat. No. 760402). Anti-human GDNF antibody (purple squares, clone A16099G) inhibits the binding in a dose-dependent manner while the isotype control (grey triangles, Cat. No. 401215) does not have the effect. This antibody blocks the binding of 0.1 µg/mL recombinant human GFRα1 protein to 0.5 µg/mL of immobilized recombinant human GDNF with an ND50 = 0.05 - 0.4 µg/mL.
Cat # Size Price Quantity Avail. Save
670903 100 µg 196€
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670904 1 mg 520€
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Description

Glial cell-derived neurotrophic factor (GDNF) is a member of the TGF-β superfamily and was first identified as a growth factor supporting the survival of embryonic midbrain neurons. GDNF has been shown to promote the development, differentiation, and survival of dopaminergic neurons by signaling through GDNF family receptor α-1 (GFRα1) and the coreceptor RET receptor tyrosine kinase. Dysregulated expression of GDNF has been reported to be associated with several mental diseases, which include depression and schizophrenia. GDNF is considered as a promising therapeutic agent for the treatment of Parkinson's disease because it can protect dopaminergic neurons from neurotoxins and promote neuron regeneration after injury. In addition to the functions in the nervous system, GDNF is also required for the differentiation of spermatogonia and kidney development. Upregulated expression of GDNF was observed in various cancer cell lines and correlates with malignant cancer progression, suggesting a role of GDNF in carcinogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human GDNF
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding between recombinant human GFRα1 and recombinant human GDNF (Cat. No. 760402). The ND50 range is 0.05 - 0.4 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820112 (BioLegend Cat. No. 670903)
AB_2820113 (BioLegend Cat. No. 670904)

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

GDNF is a secreted protein that is widely expressed in the developing and adult central nervous system.

Function
GDNF is a neurotrophic factor that promotes the development, differentiation, and the survival of dopaminergic neurons. It also participates in the recovery process after brain surgery.
Interaction
GDNF targets the neuronal cells expressing GDNF family receptors.
Ligand/Receptor
GDNF interacts with GDNF family receptor α-1 (GFRα1) and has a lower affinity with GFRα2, GFRα3, and GFRα4.
Cell Type
Embryonic Stem Cells, Mature Neurons, Neural Stem Cells, Neurons, Schwann Cells
Biology Area
Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Growth Factors
Antigen References
  1. Allen SJ, et al. 2013. Pharmacol Ther. 138:155.
  2. Hendrich J, et al. 2012. Neuroscience. 219:204.
  3. Hidalgo-Figueroa M, et al. 2012. J Neurosci. 32:864.
  4. Pascual A, et al. 2011. J Mol Endocrinol. 3:R83-92.
  5. Carnicella S, et al. 2009. Pharmacol Ther. 122:9.
  6. Sariola H, et al. 2003. J Cell Sci. 116:3855.
Gene ID
2668 View all products for this Gene ID
UniProt
View information about GDNF on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 12/13/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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