Ultra-LEAF™ Purified anti-Activin A Antibody

Pricing & Availability
Clone
A15095A (See other available formats)
Other Names
Inhibin BA (INHBA), Inhibin B-1, Follicule stimulating hormone-releasing protein (FRP), FSH releasing protein, FSH releasing factor, Erythroid differentiation factor (EDF)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
A15095A_ULEAF_PURE_Activin_A_Antibody_BA_122216
Recombinant human Activin A (grey triangles) inhibits the proliferation of mouse plasmacytoma cell line MPC 11. Anti-human Activin A antibody (clone A15095A, purple squares) inhibits the effect of recombinant Activin A (at 30 ng/ml) in MPC 11 cells in a dose dependent manner. The ND50 = 0.35 - 1.05 µg/ml.
  • A15095A_ULEAF_PURE_Activin_A_Antibody_BA_122216
    Recombinant human Activin A (grey triangles) inhibits the proliferation of mouse plasmacytoma cell line MPC 11. Anti-human Activin A antibody (clone A15095A, purple squares) inhibits the effect of recombinant Activin A (at 30 ng/ml) in MPC 11 cells in a dose dependent manner. The ND50 = 0.35 - 1.05 µg/ml.
Cat # Size Price Quantity Avail. Save
693603 100 µg 156€
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693604 1 mg 520€
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Description

Activin A was initially identified in ovarian fluid as a stimulator of FSH secretion from anterior pituitary cells. Activin A is a member of the TGFb1 family which includes TGFs, Activins, Nodal, growth and differentiation factors (GDF), and bone morphogenetic proteins (BMPs). Activin A is a dimer of the beta A subunits of human inhibin; the beta A subunit binds the beta B subunit of inhibin to form Activin AB. The beta B subunit also exists as a heterodimer to form Activin B. Activin A binds two type I and two type II serine/threonine kinase receptors at the same time. The type II receptor kinase is constitutively active and phosphorylates the type I receptor after the formation of the ligand-receptor complex. Five type II receptors have been identified: ActRIIA, ActRIIB, BMPRII, TGFβRII, and MISRII. ActRIIB binds to Activin A, BMP-2, BMP-7, GDF-8, and GDF-11. The type I receptors are named activin receptor-like kinases 1 to 7 (ALK1~7), and Activins bind to ALK4 and ALK7. Follistatin (FS) is a natural Activin A antagonist; FS neutralizes its biologic activities by preventing Activin A interaction with its receptors. Activin A has pleiotropic functions. It is a morphogen during embryonal development, regulating chondrogenic and osteogenic differentiation of mesenchymal progenitor cells (MPC). Activin A has been associated with neuroprotection, apoptosis, and fibrosis. Also, high levels of Activin A have been found in synovial fluid from patients with rheumatoid arthritis, inflammatory bowel disease, and gout. In addition, it has been speculated that Activin A, in collaboration with TGFb1 and IL-25, promotes TH9 generation and the development of allergic pathology.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Activin A.
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the inhibition of proliferation induced by human recombinant Activin A (30 ng/ml) on mouse plasmacytoma cell line MPC 11. ND50 range:  0.35 – 1.05 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2632840 (BioLegend Cat. No. 693603)
AB_2632841 (BioLegend Cat. No. 693604)

Antigen Details

Structure
Homodimer.
Distribution

BM-derived stromal fibroblasts, aortic smooth muscle cells, pulmonary epithelium, and CD4 T cells.

Function
Activin A plays a key role in embryogenesis, neointimal formation after arterial injury, early stages of chondrogenesis, osteogenesis, and hemopoiesis. Activin A drives TH9 differentiation in vitro. It is induced by Angiotensin II and alpha Thrombin and regulated by Follistatin. Activin A is induced by inflammatory cytokines and inhibited by glucocorticoids.
Interaction
The receptors for Activin A are expressed by many stromal cells, among them fibroblasts, hepatocytes, myometral cells, vascular endothelial cells, and neuronal progenitor cells.
Ligand/Receptor
Type I receptors ALK4 and ALK7, type II receptors ActRIIA and ActRIIB.
Cell Type
Fibroblasts, Epithelial cells, T cells
Biology Area
Cell Biology
Antigen References

1. Schwall RH, et al. 1988. Mol. Endocrinol. 2:1237.
2. Hashimoto M, et al. 1992. J. Biol. Chem. 267:4999.
3. Shav-Tal Y, Zipori D. 2002. Stem Cells 20:493.
4. Dohi T, et al. 2005. Gastroenterology 128:411.
5. Tsuchida K, et al. 2008. Endocr. J. 55:11.
6. Djouad F, et al. 2010. Stem Cell Research 1:11.
7. Sako D, et al. 2010. J. Biol. Chem. 285:21037.
8. Jones CP, et al. 2012. J. Allergy Clin. Immunol. 129:1000. 

Gene ID
3624 View all products for this Gene ID
UniProt
View information about Activin A on UniProt.org

Related FAQs

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 12/27/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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