Loading...

True-Nuclear™ Transcription Factor Buffer Set

Pricing & Availability
Regulatory Status
RUO
Other Names
Transcription Factor Fixation/Permeabilization Buffer Set
Ave. Rating
Submit a Review
Product Citations
publications
Transcription_Factor_F_P_Buffer_1_ICFC_012915
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_1_ICFC_012915
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • Transcription_Factor_F_P_Buffer_2_ICFC_012915
Cat # Size Price Quantity Check Availability Save
424401 120 tests 132€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

BioLegend's True-Nuclear™ Transcription Factor Buffer Set has been specially formulated for intracellular staining with minimum effect on the surface fluorochrome staining.

See the advantages of True-Nuclear™ Transcription Factor Buffer Set in this brief webinar: biolegend.com/true_nuclear_webinar.


 

Caution: The True-Nuclear™ Transcription Factor Buffer Set contains paraformaldehyde, which is toxic and mutagenic. Please handle with caution. Wear gloves, lab coats, and necessary protection to avoid direct contact. 

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Prepare fresh Transcription Factor Fix working solution by diluting the 4X Fix Concentrate (1 part) with the Fix Diluent (3 parts). 1 mL of the 1X Fix working solution is needed for each tube.

Prepare a 1X working solution of the Perm Buffer by diluting the 10X Perm Buffer with distilled water. 6.5 mL of 1X Perm Buffer is needed for each sample of tube.

NOTE: The 10X Perm Buffer may have crystallization or precipitation when it is stored at 2-8°C; however, this is normal and does not affect the buffer's performance. If there is a heavy precipitation observed after dilution to 1X working solution, it can be filtered to clarify the solution.

Application Notes

Buffer Set Contents:

  • True-Nuclear™ 4X Fix Concentrate (30 mL)
  • True-Nuclear™ Fix Diluent (100 mL)
  • True-Nuclear™ 10X Perm Buffer (100 mL)

 

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells, to detect transcription factors, and to study TIGIT in our Scientific Poster Library.

Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Manso BA, et al. 2021. STAR Protoc. 2:100485. PubMed
  3. Wu HJ, et al. 2021. Nat Commun. 12:4897. PubMed
  4. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  5. Ogawa T, et al. 2020. J Immunol. 205:907. PubMed
  6. Wang L, et al. 2019. Front Pharmacol. 1.342361111. PubMed
  7. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  8. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  9. Bujanover N, et al. 2021. Stem Cell Reports. 16:1884. PubMed
  10. Kim D, et al. 2021. Nanomicro Lett. 13:31. PubMed
  11. Zhang M, et al. 2020. Nat Commun. 4.707638889. PubMed
  12. Baram T, et al. 2021. Cells. 10:. PubMed
  13. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  14. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  15. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  16. Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
  17. Guo R, et al. 2020. Nat Microbiol. 0.938194444. PubMed
  18. van der Heiden M, et al. 2020. Scand J Immunol. e13005:. PubMed
  19. Fronik P, et al. 2021. J Med Chem. 64:12132. PubMed
  20. Webster MR, et al. 2020. Mol Cell. 77:633. PubMed
  21. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  22. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  23. Woodruff MC, et al. 2020. Nat Immunol. 1.920833333. PubMed
  24. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  25. Cortez JT, et al. 2020. Nature. 582:416. PubMed
  26. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  27. Noman MZ, et al. 2020. Sci Adv. 6:eaax7881. PubMed
  28. Almeida AS, et al. 2021. Bio Protoc. 11:e4012. PubMed
  29. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  30. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  31. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  32. Tian X, et al. 2021. Annals of Translational Medicine. 9(9):781. PubMed
  33. Wei SC, et al. 2020. Cancer Discov. . PubMed
  34. Qi X, et al. 2019. Nat Med. 25:1225. PubMed
  35. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  36. Freitas JT, et al. 2021. Pigment Cell Melanoma Res. 34:1084. PubMed
  37. Adamik J, et al. 2022. Nat Commun. 13:5184. PubMed
  38. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  39. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  40. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  41. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  42. Swartz AM, et al. 2022. Curr Protoc. 2:e410. PubMed
  43. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  44. Jarjour NN, et al. 2019. Nat Immunol. 1.310416667. PubMed
  45. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  46. Pucella JN, et al. 2019. J Immunol. 202:1383. PubMed
  47. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  48. Woodruff M, et al. 2020. medRxiv. . PubMed
  49. Wiarda JE, et al. 2022. Life Sci Alliance. 5:. PubMed
  50. Ellis GI, et al. 2022. STAR Protoc. 3:101784. PubMed
  51. Lü X, et al. 2021. Exp Ther Med. 22:1005. PubMed
  52. Li Y, et al. 2021. Cell Death Dis. 12:1001. PubMed
  53. Hu HJ, et al. 2021. Cancer Immunol Immunother. 70:3541. PubMed
  54. Ye Z, et al. 2021. Nat Commun. 12:907. PubMed
  55. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  56. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  57. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  58. Kleiman E, et al. 2021. Sci Rep. 11:9561. PubMed
  59. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  60. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  61. Zhang R, et al. 2022. Front Pharmacol. 13:870848. PubMed
  62. Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
  63. Bin L, et al. 2017. APMIS. 10.1111/apm.12748. PubMed
  64. Saito E, et al. 2019. Biomaterials. 222:119432. PubMed
  65. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  66. de Picciotto S, et al. 2022. Nat Commun. 13:3866. PubMed
  67. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  68. Ye Z, et al. 2021. NPJ Aging Mech Dis. 7:4. PubMed
  69. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  70. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  71. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  72. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  73. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  74. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  75. Datlinger P, et al. 2017. Nat Methods. 10.1038/nmeth.4177. PubMed
  76. Jarjour NN, et al. 2020. J Immunol. 204:923. PubMed
  77. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  78. Okuma A, et al. 2017. Nat Commun.. 10.1038/s41467-017-02281-x. PubMed
  79. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  80. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  81. Capelle CM, et al. 2021. Immunohorizons. 5:711. PubMed
  82. Lone W, et al. 2021. Clin Cancer Res. 27:6039. PubMed
  83. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  84. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  85. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  86. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  87. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  88. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
  89. Feng Y, et al. 2019. Stem Cell Res Ther. 0.479166667. PubMed
  90. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  91. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  92. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  93. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  94. Andricovich J, et al. 2016. J Clin Invest. 126:905-20. PubMed
  95. Yang X, et al. 2021. Bioact Mater. 3150:6. PubMed
  96. Zhu P, et al. 2022. Cell Commun Signal. 20:121. PubMed
  97. Zhao J, et al. 2021. Front Oncol. 10:621092. PubMed
  98. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  99. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  100. Gonzalez MM, et al. 2021. J Clin Invest. 131:. PubMed
  101. Moritsch S, et al. 2022. Oncoimmunology. 11:2127271. PubMed
  102. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
  103. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  104. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  105. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed

Antigen Details

Cell Type
Tregs
Biology Area
Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
NA

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 05/20/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account